ArticleOpen biology2012
The Saccharomyces cerevisiae W303-K6001 cross-platform genome sequence: insights into ancestry and physiology of a laboratory mutt.
Article in Open biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
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- Extremophilic Yeasts as Next-Generation Eukaryotic Models: Mechanisms of Stress Integration, Systems Biology and Biotechnological Applications: A Review.Journal of fungi (Basel, Switzerland) · 2026Review
- Capturing Translation in Action with Protein Synthesis Profiling.bioRxiv : the preprint server for biology · 2025Article
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- Mitochondria-plasma membrane contact sites regulate the ER-mitochondria encounter structure.Journal of cell science · 2025Article
- Spermidine toxicity in Saccharomyces cerevisiae due to mitochondrial complex III deficiency.Biogerontology · 2025Article
- Mining yeast diversity unveils novel targets for improved heterologous laccase production in Saccharomyces cerevisiae.Microbial cell factories · 2025Article
- A novel plasmid-based experimental system in Saccharomyces cerevisiae that enables the introduction of 10 different plasmids into cells.FEBS open bio · 2024Article
- The deubiquitinase Ubp3/Usp10 constrains glucose-mediated mitochondrial repression via phosphate budgeting.eLife · 2024Article
- Genetically Encoded, Noise-Tolerant, Auxin Biosensors in Yeast.ACS synthetic biology · 2024Article
- Mitochondria-ER-PM contacts regulate mitochondrial division and PI(4)P distribution.The Journal of cell biology · 2024Article
- A dynamic network model predicts the phenotypes of multicellular clusters from cellular properties.Current biology : CB · 2024Article
- Arctigenin fromAntioxidants (Basel, Switzerland) · 2024Article
- Genome-scale chromatin binding dynamics of RNA Polymerase II general transcription machinery components.The EMBO journal · 2024Article
- Understanding the molecular mechanisms of human diseases: the benefits of fission yeasts.Microbial cell (Graz, Austria) · 2024Article
- Quantifying turgor pressure in budding and fission yeasts based upon osmotic properties.Molecular biology of the cell · 2023Article
- Multicellular growth as a dynamic network of cells.bioRxiv : the preprint server for biology · 2023Article
- Comparative Proteomic Analysis of Two Commonly Used Laboratory Yeast Strains: W303 and BY4742.Proteomes · 2023Article
12 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
Saccharomyces cerevisiae strain W303 is a widely used model organism. However, little is known about its genetic origins, as it was created in the 1970s from crossing yeast strains of uncertain genealogy. To obtain insights into its ancestry and physiology, we sequenced the genome of its variant W303-K6001, a yeast model of ageing research. The combination of two next-generation sequencing (NGS) technologies (Illumina and Roche/454 sequencing) yielded an 11.8 Mb genome assembly at an N50 contig length of 262 kb. Although sequencing was substantially more precise and sensitive than whole-genome tiling arrays, both NGS platforms produced a number of false positives. At a 378× average coverage, only 74 per cent of called differences to the S288c reference genome were confirmed by both techniques. The consensus W303-K6001 genome differs in 8133 positions from S288c, predicting altered amino acid sequence in 799 proteins, including factors of ageing and stress resistance. The W303-K6001 (85.4%) genome is virtually identical (less than equal to 0.5 variations per kb) to S288c, and thus originates in the same ancestor. Non-S288c regions distribute unequally over the genome, with chromosome XVI the most (99.6%) and chromosome XI the least (54.5%) S288c-like. Several of these clusters are shared with Σ1278B, another widely used S288c-related model, indicating that these strains share a second ancestor. Thus, the W303-K6001 genome pictures details of complex genetic relationships between the model strains that date back to the early days of experimental yeast genetics. Moreover, this study underlines the necessity of combining multiple NGS and genome-assembling techniques for achieving accurate variant calling in genomic studies.
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