ArticleThe Journal of biological chemistry2012
Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Transcytosis of LDL Across Arterial Endothelium: Mechanisms and Therapeutic Targets.Arteriosclerosis, thrombosis, and vascular biology · 2025Review
- Identification of crucial LncRNAs associated with testicular development and LOC108635509 as a potential regulator in black goat spermatogenesis.BMC genomics · 2024Article
- Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters.BMC genomics · 2024Article
- Dynamin1 long- and short-tail isoforms exploit distinct recruitment and spatial patterns to form endocytic nanoclusters.Nature communications · 2024Article
- Assessment of the impact of direct in vitro PFAS treatment on mouse spermatozoa.Reproduction & fertility · 2024Article
- Investigation of Copy Number Variations (CNVs) of the GoatAnimals : an open access journal from MDPI · 2022Article
- A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse.Human genetics · 2021Review
- Mechanistic Insight into the Regulation of Lipoxygenase-Driven Lipid Peroxidation Events in Human Spermatozoa and Their Impact on Male Fertility.Antioxidants (Basel, Switzerland) · 2020Article
- Article
- Actin and Myosin in Non-Neuronal Exocytosis.Cells · 2020Review
- Whole-body exposures to radiofrequency-electromagnetic energy can cause DNA damage in mouse spermatozoa via an oxidative mechanism.Scientific reports · 2019Article
- GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse.BMC biology · 2019Article
- Mechanisms of tethering and cargo transfer during epididymosome-sperm interactions.BMC biology · 2019Article
- A Kinase Anchor Protein 4 Is Vulnerable to Oxidative Adduction in Male Germ Cells.Frontiers in cell and developmental biology · 2019Article
- Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes.Scientific reports · 2018Article
- Dynamin 2 is essential for mammalian spermatogenesis.Scientific reports · 2016Article
- Progesterone-induced Acrosome Exocytosis Requires Sequential Involvement of Calcium-independent Phospholipase A2β (iPLA2β) and Group X Secreted Phospholipase A2 (sPLA2).The Journal of biological chemistry · 2016Article
- Characterization of VAMP2 in Schistosoma japonicum and the Evaluation of Protective Efficacy Induced by Recombinant SjVAMP2 in Mice.PloS one · 2015Article
- Unresolved questions concerning mammalian sperm acrosomal exocytosis.Biology of reproduction · 2014Review
- Synthesis of Dynole 34-2, Dynole 2-24 and Dyngo 4a for investigating dynamin GTPase.Nature protocols · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian spermatozoa must complete an acrosome reaction prior to fertilizing an oocyte. The acrosome reaction is a unique exocytotic event involving a series of prolonged membrane fusions that ultimately result in the production of membrane vesicles and release of the acrosomal contents. This event requires the concerted action of a large number of fusion-competent signaling and scaffolding proteins. Here we show that two different members of the dynamin GTPase family localize to the developing acrosome of maturing mouse germ cells. Both dynamin 1 and 2 also remain within the periacrosomal region of mature mouse spermatozoa and are thus well positioned to regulate the acrosome reaction. Two pharmacological inhibitors of dynamin, dynasore and Dyngo-4a, blocked the in vitro induction of acrosomal exocytosis by progesterone, but not by the calcium ionophore A23187, and elicited a concomitant reduction of in vitro fertilization. In vivo treatment with these inhibitors also resulted in spermatozoa displaying reduced acrosome reaction potential. Dynamin 1 and 2 phosphorylation increased on progesterone treatment, and this was also selectively blocked by dynasore. On the basis of our collective data, we propose that dynamin could regulate specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.