ArticleThe Journal of biological chemistry2012
An interaction between DNA polymerase and helicase is essential for the high processivity of the bacteriophage T7 replisome.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Real-time imaging of rotation during synthesis by the replisome.Science advances · 2026Article
- IBPred: A sequence-based predictor for identifying ion binding protein in phage.Computational and structural biotechnology journal · 2022Article
- Article
- DNA replication machinery: Insights fromComputational and structural biotechnology journal · 2021Review
- Review
- Combined Solution and Crystal Methods Reveal the Electrostatic Tethers That Provide a Flexible Platform for Replication Activities in the Bacteriophage T7 Replisome.Biochemistry · 2019Article
- Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B.BMC plant biology · 2019Article
- Helicase promotes replication re-initiation from an RNA transcript.Nature communications · 2018Article
- Plant organellar DNA primase-helicase synthesizes RNA primers for organellar DNA polymerases using a unique recognition sequence.Nucleic acids research · 2017Article
- Cryo-EM structure of the replisome reveals multiple interactions coordinating DNA synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Hybrid Methods Reveal Multiple Flexibly Linked DNA Polymerases within the Bacteriophage T7 Replisome.Structure (London, England : 1993) · 2017Article
- T7 replisome directly overcomes DNA damage.Nature communications · 2015Article
- Cooperative base pair melting by helicase and polymerase positioned one nucleotide from each other.eLife · 2015Article
- Uniqueness of models from small-angle scattering data: the impact of a hydration shell and complementary NMR restraints.Acta crystallographica. Section D, Biological crystallography · 2015Article
- A Replisome's journey through the bacterial chromosome.Frontiers in microbiology · 2015Review
- Helicase and polymerase move together close to the fork junction and copy DNA in one-nucleotide steps.Cell reports · 2014Article
- Single-molecule studies of polymerase dynamics and stoichiometry at the bacteriophage T7 replication machinery.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bacteriophage T7 DNA polymerase - sequenase.Frontiers in microbiology · 2014Review
- Discrete interactions between bacteriophage T7 primase-helicase and DNA polymerase drive the formation of a priming complex containing two copies of DNA polymerase.Biochemistry · 2013Article
- Impact of macromolecular crowding on DNA replication.Nature communications · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Synthesis of the leading DNA strand requires the coordinated activity of DNA polymerase and DNA helicase, whereas synthesis of the lagging strand involves interactions of these proteins with DNA primase. We present the first structural model of a bacteriophage T7 DNA helicase-DNA polymerase complex using a combination of small angle x-ray scattering, single-molecule, and biochemical methods. We propose that the protein-protein interface stabilizing the leading strand synthesis involves two distinct interactions: a stable binding of the helicase to the palm domain of the polymerase and an electrostatic binding of the carboxyl-terminal tail of the helicase to a basic patch on the polymerase. DNA primase facilitates binding of DNA helicase to ssDNA and contributes to formation of the DNA helicase-DNA polymerase complex by stabilizing DNA helicase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.