ArticleJournal of translational medicine2012
Development of a new humanized mouse model to study acute inflammatory arthritis.
Article in Journal of translational medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Murine and Humanized Mouse Models in Autoimmune Disease Research and Therapeutics Development.Biology · 2026Review
- Validation of a model of rheumatoid arthritis using mice reconstituted with patient peripheral blood mononuclear cells.Disease models & mechanisms · 2025Article
- The Sunrise of Tertiary Lymphoid Structures in Cancer.Immunological reviews · 2025Review
- Humanized Mouse Models for the Study of Periodontitis: An Opportunity to Elucidate Unresolved Aspects of Its Immunopathogenesis and Analyze New Immunotherapeutic Strategies.Frontiers in immunology · 2021Review
- Humanized Mice for Live-Attenuated Vaccine Research: From Unmet Potential to New Promises.Vaccines · 2020Review
- Human immune cells infiltrate the spinal cord and impair recovery after spinal cord injury in humanized mice.Scientific reports · 2019Article
- The development of human immune system mice and their use to study tolerance and autoimmunity.Journal of translational autoimmunity · 2019Review
- Anti-inflammatory Activity of MTL-CEBPA, a Small Activating RNA Drug, in LPS-Stimulated Monocytes and Humanized Mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Humanized Mouse Models of Rheumatoid Arthritis for Studies on Immunopathogenesis and Preclinical Testing of Cell-Based Therapies.Frontiers in immunology · 2019Review
- Humanized Mouse Models of Clinical Disease.Annual review of pathology · 2017Review
- Development of the Digital Arthritis Index, a Novel Metric to Measure Disease Parameters in a Rat Model of Rheumatoid Arthritis.Frontiers in pharmacology · 2017Article
- Article
- Mice engrafted with human hematopoietic stem cells support a human myeloid cell inflammatory response in vivo.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2016Article
- Use of Humanized Mice to Study the Pathogenesis of Autoimmune and Inflammatory Diseases.Inflammatory bowel diseases · 2015Review
- Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis.BioMed research international · 2015Article
- Mesenchymal properties of SJL mice-stem cells and their efficacy as autologous therapy in a relapsing-remitting multiple sclerosis model.Stem cell research & therapy · 2014Article
- TNF-related apoptosis-inducing ligand (TRAIL) in rheumatoid arthritis: what's new?Clinical and experimental medicine · 2014Review
- Novel pyridazinone inhibitors for vascular adhesion protein-1 (VAP-1): old target-new inhibition mode.Journal of medicinal chemistry · 2013Article
- High-resolution magnetic resonance imaging of ankle joints in murine arthritis discriminates inflammation and bone destruction in a quantifiable manner.Arthritis and rheumatism · 2013Article
- Humanizing the mouse: in defense of murine models of critical illness.American journal of respiratory and critical care medicine · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundSubstantial advances have been generated in understanding the pathogenesis of rheumatoid arthritis (RA). Current murine models of RA-like disease have provided great insights into the molecular mechanism of inflammatory arthritis due to the use of genetically deficient or transgenic mice. However, these studies are limited by differences that exist between human and murine immune systems. Thus, the development of an animal model that utilizes human immune cells, will afford the opportunity to study their function in the initiation and propagation of inflammatory arthritis.
methodsOne to two-day old irradiated NOD-scid IL2rγ(null) (NSG) mice were reconstituted with human CD34+ cord blood stem cells. Leukocytes were analyzed by flow cytometry and circulating antibodies were determined by ELISA. Arthritis was induced by injecting complete Freund's adjuvant into knee or ankle joints. Mice were also treated with the TNF inhibitor, Etanercept, or PBS and joints were analyzed histologically.
resultsHumanized mice were established with high reconstitution rates and were able to spontaneously produce human immunoglobulins as well as specific IgG in response to immunization. Intraperitoneal injection of thioglycolate or injection of complete Freund's adjuvant into joints resulted in migration of human immune cells to the injected sites. Arthritic humanized mice treated with Etanercept had markedly less inflammation, which was associated with decreased total numbers of human CD45+ cells, including human lymphocytes and neutrophils.
conclusionsThe humanized mouse model is a new model to study inflammatory arthritis disease using human leukocytes without rejection of engrafted tissue. Future studies may adapt this system to incorporate RA patient cord blood and develop a chimeric animal model of inflammatory arthritis using genetically predisposed immune cells.
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