ArticleBMC genomics2012
In depth comparison of an individual's DNA and its lymphoblastoid cell line using whole genome sequencing.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- De novo single-nucleotide and copy number variation in discordant monozygotic twins reveals disease-related genes.European journal of human genetics : EJHG · 2019Trial
- Copy number variant scores are associated with cerebrovascular pathology in aging.Molecular psychiatry · 2026Article
- IGLoo enables comprehensive analysis and assembly of immunoglobulin heavy-chain loci in lymphoblastoid cell lines using PacBio high-fidelity reads.Cell reports methods · 2025Article
- Y chromosome sequence and epigenomic reconstruction across human populations.Communications biology · 2023Article
- A high-throughput real-time PCR tissue-of-origin test to distinguish blood from lymphoblastoid cell line DNA for (epi)genomic studies.Scientific reports · 2022Article
- Comparison of mitochondrial DNA sequences from whole blood and lymphoblastoid cell lines.Scientific reports · 2022Article
- Discovery of genomic variation across a generation.Human molecular genetics · 2021Article
- Limitations of lymphoblastoid cell lines for establishing genetic reference datasets in the immunoglobulin loci.PloS one · 2021Article
- Diagnosing Cornelia de Lange syndrome and related neurodevelopmental disorders using RNA sequencing.Genetics in medicine : official journal of the American College of Medical Genetics · 2020Article
- Extensive epigenetic and transcriptomic variability between genetically identical human B-lymphoblastoid cells with implications in pharmacogenomics research.Scientific reports · 2019Article
- Investigating mitonuclear interactions in human admixed populations.Nature ecology & evolution · 2019Article
- The Malaria-Protective Human Glycophorin Structural Variant DUP4 Shows Somatic Mosaicism and Association with Hemoglobin Levels.American journal of human genetics · 2018Article
- Genome sequencing uncovers phenocopies in primary progressive multiple sclerosis.Annals of neurology · 2018Article
- Oxidative Stress Challenge Uncovers Trichloroacetaldehyde Hydrate-Induced Mitoplasticity in Autistic and Control Lymphoblastoid Cell Lines.Scientific reports · 2017Article
- Modulation of Immunological Pathways in Autistic and Neurotypical Lymphoblastoid Cell Lines by the Enteric Microbiome Metabolite Propionic Acid.Frontiers in immunology · 2017Article
- Differences between the genomes of lymphoblastoid cell lines and blood-derived samples.Advances in genomics and genetics · 2017Article
- Modulation of mitochondrial function by the microbiome metabolite propionic acid in autism and control cell lines.Translational psychiatry · 2016Article
- Integrity of genome-wide genotype data from low passage lymphoblastoid cell lines.Genomics data · 2016Article
- A pilot study on commonality and specificity of copy number variants in schizophrenia and bipolar disorder.Translational psychiatry · 2016Article
- Transcriptome outlier analysis implicates schizophrenia susceptibility genes and enriches putatively functional rare genetic variants.Human molecular genetics · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundA detailed analysis of whole genomes can be now achieved with next generation sequencing. Epstein Barr Virus (EBV) transformation is a widely used strategy in clinical research to obtain an unlimited source of a subject's DNA. Although the mechanism of transformation and immortalization by EBV is relatively well known at the transcriptional and proteomic level, the genetic consequences of EBV transformation are less well understood. A detailed analysis of the genetic alterations introduced by EBV transformation is highly relevant, as it will inform on the usefulness and limitations of this approach.
resultsWe used whole genome sequencing to assess the genomic signature of a low-passage lymphoblastoid cell line (LCL). Specifically, we sequenced the full genome (40X) of an individual using DNA purified from fresh whole blood as well as DNA from his LCL. A total of 217.33 Gb of sequence were generated from the cell line and 238.95 Gb from the normal genomic DNA. We determined with high confidence that 99.2% of the genomes were identical, with no reproducible changes in structural variation (chromosomal rearrangements and copy number variations) or insertion/deletion polymorphisms (indels).
conclusionsOur results suggest that, at this level of resolution, the LCL is genetically indistinguishable from its genomic counterpart and therefore their use in clinical research is not likely to introduce a significant bias.
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