ArticleMicrobial cell factories2012
Role of bicarbonate as a pH buffer and electron sink in microbial dechlorination of chloroethenes.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Biotransforming the "Forever Chemicals": Trends and Insights from Microbiological Studies on PFAS.Environmental science & technology · 2025Review
- Mixotrophy in Marine Microalgae to Enhance Their Bioactivity.Microorganisms · 2025Article
- Acetylene Tunes Microbial Growth During Aerobic Cometabolism of Trichloroethene.Environmental science & technology · 2024Article
- Interaction between nitrate and trichloroethene bioreduction in mixed anaerobic cultures.Frontiers in microbiology · 2024Article
- Microbial hydrogen consumption leads to a significant pH increase under high-saline-conditions: implications for hydrogen storage in salt caverns.Scientific reports · 2023Article
- Decoupling FeEnvironmental science & technology · 2023Article
- An Ion Chromatography Method for Simultaneous Quantification of Chromate, Arsenate, Selenate, Perchlorate, and Other Inorganic Anions in Environmental Media.Environmental engineering science · 2021Article
- The occurrence and ecology of microbial chain elongation of carboxylates in soils.The ISME journal · 2021Article
- Safe-by-Design CuO Nanoparticles via Fe-Doping, Cu-O Bond Length Variation, and Biological Assessment in Cells and Zebrafish Embryos.ACS nano · 2017Article
- The effects of COBiotechnology for biofuels · 2017Article
- Effects of 1,1,1-Trichloroethane and Triclocarban on Reductive Dechlorination of Trichloroethene in a TCE-Reducing Culture.Frontiers in microbiology · 2017Article
- Formate-Dependent Microbial Conversion of CO2 and the Dominant Pathways of Methanogenesis in Production Water of High-temperature Oil Reservoirs Amended with Bicarbonate.Frontiers in microbiology · 2016Article
- The roles of methanogens and acetogens in dechlorination of trichloroethene using different electron donors.Environmental science and pollution research international · 2015Article
- Use of silicate minerals for pH control during reductive dechlorination of chloroethenes in batch cultures of different microbial consortia.Applied and environmental microbiology · 2014Article
- Selective enrichment yields robust ethene-producing dechlorinating cultures from microcosms stalled at cis-dichloroethene.PloS one · 2014Article
- Article
- Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities.mSphereArticle
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBuffering to achieve pH control is crucial for successful trichloroethene (TCE) anaerobic bioremediation. Bicarbonate (HCO3-) is the natural buffer in groundwater and the buffer of choice in the laboratory and at contaminated sites undergoing biological treatment with organohalide respiring microorganisms. However, HCO3- also serves as the electron acceptor for hydrogenotrophic methanogens and hydrogenotrophic homoacetogens, two microbial groups competing with organohalide respirers for hydrogen (H2). We studied the effect of HCO3- as a buffering agent and the effect of HCO3--consuming reactions in a range of concentrations (2.5-30 mM) with an initial pH of 7.5 in H2-fed TCE reductively dechlorinating communities containing Dehalococcoides, hydrogenotrophic methanogens, and hydrogenotrophic homoacetogens.
resultsRate differences in TCE dechlorination were observed as a result of added varying HCO3- concentrations due to H2-fed electrons channeled towards methanogenesis and homoacetogenesis and pH increases (up to 8.7) from biological HCO3- consumption. Significantly faster dechlorination rates were noted at all HCO3- concentrations tested when the pH buffering was improved by providing 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) as an additional buffer. Electron balances and quantitative PCR revealed that methanogenesis was the main electron sink when the initial HCO3- concentrations were 2.5 and 5 mM, while homoacetogenesis was the dominant process and sink when 10 and 30 mM HCO3- were provided initially.
conclusionsOur study reveals that HCO3- is an important variable for bioremediation of chloroethenes as it has a prominent role as an electron acceptor for methanogenesis and homoacetogenesis. It also illustrates the changes in rates and extent of reductive dechlorination resulting from the combined effect of electron donor competition stimulated by HCO3- and the changes in pH exerted by methanogens and homoacetogens.
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