ArticleG3 (Bethesda, Md.)2012
Using near-infrared spectroscopy to resolve the species, gender, age, and the presence of Wolbachia infection in laboratory-reared Drosophila.
Article in G3 (Bethesda, Md.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- First report using near-infrared spectroscopy to age-grade Phlebotomus papatasi [Scopoli, 1786] sand flies.Scientific reports · 2025Article
- Age, sex, and mating status discrimination in the sand fly Lutzomyia longipalpis using near infra-red spectroscopy (NIRS).Parasites & vectors · 2024Article
- Article
- Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique.Parasite (Paris, France) · 2023Article
- Rapid and Non-Invasive Detection ofViruses · 2022Article
- Near infrared spectroscopy accurately detects Trypanosoma cruzi non-destructively in midguts, rectum and excreta samples of Triatoma infestans.Scientific reports · 2021Article
- High throughput estimates of Wolbachia, Zika and chikungunya infection in Aedes aegypti by near-infrared spectroscopy to improve arbovirus surveillance.Communications biology · 2021Article
- Near-infrared spectroscopy for metabolite quantification and species identification.Ecology and evolution · 2019Article
- Proximal Remote Sensing to Non-destructively Detect and Diagnose Physiological Responses by Host Insect Larvae to Parasitism.Frontiers in physiology · 2018Article
- Analysis of near infrared spectra for age-grading of wild populations of Anopheles gambiae.Parasites & vectors · 2017Article
- Article
- Rapid and Non-destructive Detection and Identification of Two Strains of Wolbachia in Aedes aegypti by Near-Infrared Spectroscopy.PLoS neglected tropical diseases · 2016Article
- Near Infrared Imaging As a Method of Studying Tsetse Fly (Diptera: Glossinidae) Pupal Development.Journal of insect science (Online) · 2016Article
- Detection of temporal changes in insect body reflectance in response to killing agents.PloS one · 2015Article
- A near-infrared spectroscopy routine for unambiguous identification of cryptic ant species.PeerJ · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the study was to determine the accuracy of near-infrared spectroscopy (NIRS) in determining species, gender, age, and the presence of the common endosymbiont Wolbachia in laboratory-reared Drosophila. NIRS measures the absorption of light by organic molecules. Initially, a calibration model was developed for each study. An independent set with flies not involved in initial cross-validation was then used to validate the accuracy of each calibration model. Flies from the independent sets were correctly classified into Drosophila melanogaster and Drosophila simulans with 94% and 82% accuracy, respectively, whereas flies were successfully classified by gender with accuracy greater than 90%. In the age grading test, correlation plots of the actual and predicted age for males and females of D. melanogaster and D. simulans were shown to be overlapping between the adjacent age groups. It is, however, possible to predict the age of flies as less than 9 days of age with 62-88% accuracy and flies that are equal to or older than 9 days of age with 91-98% accuracy. Finally, we used NIRS to detect the presence of Wolbachia in flies. Flies from the independent sets were successfully identified as infected or not infected with Wolbachia with approximately 90% accuracy. These results suggest that NIRS has the potential to quantify the species, gender, and presence of Wolbachia in fly populations. However, additional optimization of the protocol may be necessary before the technique can reliably estimate fly age.
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