ArticleIranian journal of microbiology2012
Characterization of Shiga toxin-producing Escherichia coli (STEC) in feces of healthy and diarrheic calves in Urmia region, Iran.
Article in Iranian journal of microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- How to Improve Surveillance Program for Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2024Article
- RETRACTED ARTICLE: Molecular detection of pathogenic Escherichia coli strains and their antibiogram associated with risk factors from diarrheic calves in Jimma Ethiopia.Scientific reports · 2021Article
- Frequency of k99, stx1, and stx2 Virulence Factors in Escherichia coli isolated from Diarrheic and Clinically Healthy Suckling Calves in Sistan and Baluchistan Province, Iran.Archives of Razi Institute · 2021Article
- Molecular analysis of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 strains isolated from calves.The Onderstepoort journal of veterinary research · 2018Article
- Virulent gene profile and antibiotic susceptibility pattern of Shiga toxin-producing Escherichia coli (STEC) from cattle and camels in Maiduguri, North-Eastern Nigeria.Tropical animal health and production · 2018Article
- Serogroups, subtypes and virulence factors of shiga toxin-producingGastroenterology and hepatology from bed to bench · 2018Article
- Prevalence and antimicrobial resistance pattern of Shiga toxigenicVeterinary world · 2017Article
- A study on association of virulence determinants of verotoxic Escherichia coli isolated from cattle calves.Veterinary world · 2016Article
- Distribution of Shiga toxin genes subtypes in B1 phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays.Iranian journal of microbiology · 2015Article
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Abstract
BACKGROUND AND
objectivesShiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and contamination of foods of animal origin has been a major public health concern. The aim of the present study was to determine the dissemination of STEC in healthy and diarrheic calves in Urmia region which is located in West Azerbaijan province, Iran. MATERIALS AND
methodsIn the current study, a total of 124 Escherichia coli isolates from clinically healthy (n = 73) and diarrheic calves (51) belonging to 6 different farms located in West Azerbaijan province, Iran, were screened by the polymerase chain reaction (PCR) assay for the presence of virulence genes characteristic for STEC, that is, Shiga-toxin producing gene(s) (stx1, stx2), intimin (eaeA) and enterohemolysin (hlyA).
resultsSTEC isolates were recovered from 21.92% (16/73) in healthy calves, and 19.6% (10/51) in diarrheic calves. Overall, PCR results showed that 6 (23.1%) isolates carried stx1 gene, 7 (26.92%) possessed stx2 gene while 13 isolates (50%) gave positive amplicon both for stx1 and stx2 genes. All stx positive isolates were assayed further to detect eaeA and hlyA sequences. Seven out of the 26 (26.92%) Shiga toxin gene positive isolates were positive for the eaeA gene, and 15 (57.69%) were positive for the hlyA gene. Both virulence genes (eaeA and hlyA) in the same isolate were observed in 5 (19.23%) of the stx(+) isolates. In total, diverse virulence gene profiles were detected, from which isolates with the genetic profile stx1 stx2 hlyA was the most prevalent. In addition, eaeA gene was more evident in isolates from diarrheic calves than in healthy calves.
conclusionThere was no significant difference in detecting STEC isolates between healthy and diarrheic calves. It seems that calves to be the reservoir of STEC within the herds and calf management may represent specific control points for reducing STEC spread within dairy units.
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22973471PMC3434643What OpenQuestion holds
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