ArticleFrontiers in endocrinology2012
Setting Up a Bioluminescence Resonance Energy Transfer High throughput Screening Assay to Search for Protein/Protein Interaction Inhibitors in Mammalian Cells.
Article in Frontiers in endocrinology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 45 citations in OpenAlex.
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- Optimization of BRET saturation assays for robust and sensitive cytosolic protein-protein interaction studies.Scientific reports · 2022Article
- Live-Cell Analysis of Human Cytomegalovirus DNA Polymerase Holoenzyme Assembly by Resonance Energy Transfer Methods.Microorganisms · 2021Article
- Creation of different bioluminescence resonance energy transfer based biosensors with high affinity to VEGF.PloS one · 2020Article
- Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology.Molecules (Basel, Switzerland) · 2019Review
- Combining Optical Approaches with Human Inducible Pluripotent Stem Cells in G Protein-Coupled Receptor Drug Screening and Development.Biomolecules · 2018Review
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- A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging.Scientific reports · 2018Article
- Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors.Molecular biotechnology · 2018Article
- Identification of danthron as an isoform-specific inhibitor of HEME OXYGENASE-1/cytochrome P450 reductase interaction with anti-tumor activity.Journal of biomedical science · 2018Article
- Regulation of NF-κB by the p105-ABIN2-TPL2 complex and RelAp43 during rabies virus infection.PLoS pathogens · 2017Article
- Enabling systematic interrogation of protein-protein interactions in live cells with a versatile ultra-high-throughput biosensor platform.Journal of molecular cell biology · 2016Article
- NanoLuc Luciferase - A Multifunctional Tool for High Throughput Antibody Screening.Frontiers in pharmacology · 2016Article
- Target engagement and drug residence time can be observed in living cells with BRET.Nature communications · 2015Article
- Simultaneous recording of fluorescence and electrical signals by photometric patch electrode in deep brain regions in vivo.Journal of neurophysiology · 2015Article
- Targeting the cis-dimerization of LINGO-1 with low MW compounds affects its downstream signalling.British journal of pharmacology · 2015Article
- Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Protein-protein interactions of mitochondrial-associated protein via bioluminescence resonance energy transfer.Biophysics and physicobiology · 2015Review
- New pharmacological perspectives for the leptin receptor in the treatment of obesity.Frontiers in endocrinology · 2014Review
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Each step of the cell life and its response or adaptation to its environment are mediated by a network of protein/protein interactions termed "interactome." Our knowledge of this network keeps growing due to the development of sensitive techniques devoted to study these interactions. The bioluminescence resonance energy transfer (BRET) technique was primarily developed to allow the dynamic monitoring of protein/protein interactions (PPI) in living cells, and has widely been used to study receptor activation by intra- or extra-molecular conformational changes within receptors and activated complexes in mammal cells. Some interactions are described as crucial in human pathological processes, and a new class of drugs targeting them has recently emerged. The BRET method is well suited to identify inhibitors of PPI and here is described why and how to set up and optimize a high throughput screening assay based on BRET to search for such inhibitory compounds. The different parameters to take into account when developing such BRET assays in mammal cells are reviewed to give general guidelines: considerations on the targeted interaction, choice of BRET version, inducibility of the interaction, kinetic of the monitored interaction, and of the BRET reading, influence of substrate concentration, number of cells and medium composition used on the Z' factor, and expected interferences from colored or fluorescent compounds.
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