ArticleMolecular biology of the cell2012
Sub1 associates with Spt5 and influences RNA polymerase II transcription elongation rate.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- An extrinsic motor directs chromatin loop formation by cohesin.The EMBO journal · 2024Article
- Splicing-specific transcriptome-wide association uncovers genetic mechanisms for schizophrenia.American journal of human genetics · 2024Article
- Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.Biochemistry · 2023Article
- The Role ofCells · 2022Article
- The Catalytic Subunit ofInternational journal of molecular sciences · 2022Article
- RNA polymerase II speed: a key player in controlling and adapting transcriptome composition.The EMBO journal · 2021Review
- Regulation of Eukaryotic RNAPs Activities by Phosphorylation.Frontiers in molecular biosciences · 2021Review
- The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription.Nucleic acids research · 2019Article
- Sub1 and RNAPII, until termination does them part.Transcription · 2018Review
- Sub1/PC4, a multifaceted factor: from transcription to genome stability.Current genetics · 2017Review
- The histone variant H2A.Z promotes efficient cotranscriptional splicing inGenes & development · 2017Article
- Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis.Nucleic acids research · 2017Article
- Barcode Sequencing Screen Identifies SUB1 as a Regulator of Yeast Pheromone Inducible Genes.G3 (Bethesda, Md.) · 2016Article
- Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica.Scientific reports · 2016Article
- Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA.Chemical communications (Cambridge, England) · 2015Article
- Ubiquitous transcription factors display structural plasticity and diverse functions: NusG proteins - Shifting shapes and paradigms.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- SUB1 Plays a Negative Role during Starvation Induced Sporulation Program in Saccharomyces cerevisiae.PloS one · 2015Article
- Adventures in time and space: splicing efficiency and RNA polymerase II elongation rate.RNA biology · 2014Review
- Sub1 and Maf1, two effectors of RNA polymerase III, are involved in the yeast quiescence cycle.PloS one · 2014Article
- From structure to systems: high-resolution, quantitative genetic analysis of RNA polymerase II.Cell · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcriptional coactivator Sub1 has been implicated in several steps of mRNA metabolism in yeast, such as the activation of transcription, termination, and 3'-end formation. In addition, Sub1 globally regulates RNA polymerase II phosphorylation, and most recently it has been shown that it is a functional component of the preinitiation complex. Here we present evidence that Sub1 plays a significant role in transcription elongation by RNA polymerase II (RNAPII). We show that SUB1 genetically interacts with the gene encoding the elongation factor Spt5, that Sub1 influences Spt5 phosphorylation of the carboxy-terminal domain of RNAPII largest subunit by the kinase Bur1, and that both Sub1 and Spt5 copurify in the same complex, likely during early transcription elongation. Indeed, our data indicate that Sub1 influences Spt5-Rpb1 interaction. In addition, biochemical and molecular data show that Sub1 influences transcription elongation of constitutive and inducible genes and associates with coding regions in a transcription-dependent manner. Taken together, our results indicate that Sub1 associates with Spt5 and influences Spt5-Rpb1 complex levels and consequently transcription elongation rate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.