Evidence map›Paper›PMID 22973051›Full record

ArticleMolecular endocrinology (Baltimore, Md.)2012

The microRNA (miR)-199a/214 cluster mediates opposing effects of progesterone and estrogen on uterine contractility during pregnancy and labor.

Koriand'r C Williams, Nora E Renthal, Robert D Gerard, Carole R Mendelson

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular endocrinology (Baltimore, Md.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 65 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
65citing papers in PubMed, 2 pooled it
5.3field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

65 citing papers in PubMed, 2 syntheses or guidelines pooled it, 113 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Trial
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. The hormonal control of parturition.Physiological reviews · 2024
    Review
  12. Article
  13. Article
  14. Study on the Pattern of Postpartum Uterine Involution in Dairy Cows.Animals : an open access journal from MDPI · 2023
    Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review

5 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Koriand'r C WilliamsDepartment of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Nora E Renthal
Robert D Gerard
Carole R Mendelson
The University of Texas Southwestern Medical Center · US

Funding

TRANSCRIPTIONAL REGULATION OF CERVICAL COMPETENCE DURING PREGNANCYP01HD011149 · NICHD · UT SOUTHWESTERN MEDICAL CENTER · PI MENDELSON, CAROLE R · 1998 to 2015
$17.7M
MECHANISMS OF DRUG ACTION AND DISPOSITIONT32GM007062 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI MANGELSDORF, DAVID J · 1985 to 2020
$7.4M
NICHD NIH HHS 5-P01-HD11149NICHD NIH HHS P01 HD011149NIGMS NIH HHS GM007062NIGMS NIH HHS T32 GM007062
6 · The paper itself

Abstract

Progesterone (P(4)) and estradiol-17β (E(2)) play critical and opposing roles in regulating myometrial quiescence and contractility during pregnancy and labor. Although these contrasting hormonal effects are likely mediated via differential regulation of inflammatory and contractile genes, the underlying mechanisms remain incompletely understood. Recently we discovered that targets of the microRNA (miR)-200 family, transcription factors zinc finger E-box binding homeobox (ZEB)-1 and ZEB2, serve as P(4)/progesterone receptor-mediated regulators of uterine quiescence during pregnancy. In the present study, we found that levels of the clustered miRNAs, miR-199a-3p and miR-214, were significantly decreased in laboring myometrium of pregnant mice and humans and in an inflammatory mouse model of preterm labor, whereas the miR-199a-3p/miR-214 target, cyclooxygenase-2, a critical enzyme in synthesis of proinflammatory prostaglandins, was coordinately increased. Overexpression of miR-199a-3p and miR-214 in cultured human myometrial cells inhibited cyclooxygenase-2 protein and blocked TNF-α-induced myometrial cell contractility, suggesting their physiological relevance. Notably, E(2) treatment of ovariectomized mice suppressed, whereas P(4) enhanced uterine miR-199a-3p/214 expression. Intriguingly, these opposing hormonal effects were mediated by ZEB1, which is induced by P(4), inhibited by E(2) and activates miR199a/214 transcription. Together, these findings identify miR-199a-3p/miR-214 as important regulators of myometrial contractility and provide new insight into strategies to prevent preterm birth.

Indexed as

AnimalsBase SequenceCyclooxygenase 2EstrogensFemaleGene Expression RegulationHomeodomain ProteinsHumansInflammation MediatorsLabor, ObstetricMiceMice, Inbred ICRMicroRNAsMolecular Sequence DataMultigene FamilyMyometriumCyclooxygenase 2EstrogensHomeodomain ProteinsInflammation MediatorsMicroRNAsmirn199 microRNA, humanMirn199 microRNA, mouseMIRN214 microRNA, humanMirn214 microRNA, mouseProgesteroneTranscription FactorsZEB1 protein, humanZinc Finger E-box-Binding Homeobox 1

Identifiers

PMID22973051
PMCPMC3487626
OpenAlexW2136302785

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.