ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Live imaging of targeted cell ablation in Xenopus: a new model to study demyelination and repair.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Review
- Accelerated protein retention expansion microscopy using microwave radiation.Cell reports methods · 2024Article
- Accelerated protein retention expansion microscopy using microwave radiation.bioRxiv : the preprint server for biology · 2024Article
- Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS.Journal of neuroinflammation · 2023Article
- Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies.Brain : a journal of neurology · 2023Article
- Oxygen-insensitive nitroreductase E. coli NfsA, but not NfsB, is inhibited by fumarate.Proteins · 2023Article
- Structure and Dynamics of ThreeInternational journal of molecular sciences · 2023Article
- Aquatic Freshwater Vertebrate Models of Epilepsy Pathology: Past Discoveries and Future Directions for Therapeutic Discovery.International journal of molecular sciences · 2022Review
- Increased Remyelination and Proregenerative Microglia Under Siponimod Therapy in Mechanistic Models.Neurology(R) neuroimmunology & neuroinflammation · 2022Article
- Teriflunomide Promotes Oligodendroglial 8,9-Unsaturated Sterol Accumulation and CNS Remyelination.Neurology(R) neuroimmunology & neuroinflammation · 2021Article
- The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN.The Biochemical journal · 2021Article
- New Species Can Broaden Myelin Research: Suitability of Little Skate,Life (Basel, Switzerland) · 2021Article
- Xenopus leads the way: Frogs as a pioneering model to understand the human brain.Genesis (New York, N.Y. : 2000) · 2021Review
- Evolutionary Origins of the Oligodendrocyte Cell Type and Adaptive Myelination.Frontiers in neuroscience · 2021Review
- Bruton's Tyrosine Kinase Inhibition Promotes Myelin Repair.Brain plasticity (Amsterdam, Netherlands) · 2020Article
- Thyroid hormone regulation of neural stem cell fate: From development to ageing.Acta physiologica (Oxford, England) · 2020Review
- Using Xenopus oocytes in neurological disease drug discovery.Expert opinion on drug discovery · 2020Review
- Review
- Human amniotic fluid contaminants alter thyroid hormone signalling and early brain development in Xenopus embryos.Scientific reports · 2017Article
- Glial Cells and Their Function in the Adult Brain: A Journey through the History of Their Ablation.Frontiers in cellular neuroscience · 2017Review
Corrections and comments
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Authors and funding
16 authors at 7 institutions in 3 countries.
Funding
Abstract
Live imaging studies of the processes of demyelination and remyelination have so far been technically limited in mammals. We have thus generated a Xenopus laevis transgenic line allowing live imaging and conditional ablation of myelinating oligodendrocytes throughout the CNS. In these transgenic pMBP-eGFP-NTR tadpoles the myelin basic protein (MBP) regulatory sequences, specific to mature oligodendrocytes, are used to drive expression of an eGFP (enhanced green fluorescent protein) reporter fused to the Escherichia coli nitroreductase (NTR) selection enzyme. This enzyme converts the innocuous prodrug metronidazole (MTZ) to a cytotoxin. Using two-photon imaging in vivo, we show that pMBP-eGFP-NTR tadpoles display a graded oligodendrocyte ablation in response to MTZ, which depends on the exposure time to MTZ. MTZ-induced cell death was restricted to oligodendrocytes, without detectable axonal damage. After cessation of MTZ treatment, remyelination proceeded spontaneously, but was strongly accelerated by retinoic acid. Altogether, these features establish the Xenopus pMBP-eGFP-NTR line as a novel in vivo model for the study of demyelination/remyelination processes and for large-scale screens of therapeutic agents promoting myelin repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.