ArticleTransgenic research2013
Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Strategies, Achievements, and Potential Challenges of Plant and Microbial Chassis in the Biosynthesis of Plant Secondary Metabolites.Molecules (Basel, Switzerland) · 2024Review
- Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning.Frontiers in bioengineering and biotechnology · 2023Article
- Efficient assembly of long DNA fragments and multiple genes with improved nickase-based cloning and Cre/loxP recombination.Plant biotechnology journal · 2022Article
- An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants.Plants (Basel, Switzerland) · 2022Article
- Photosynthesis research under climate change.Photosynthesis research · 2021Review
- Efficient Gene Stacking in Rice Using the GAANTRY System.Rice (New York, N.Y.) · 2021Article
- Gene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY).Methods in molecular biology (Clifton, N.J.) · 2021Article
- Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants.PloS one · 2018Article
- Article
- Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems.Plant molecular biology · 2016Article
- Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana.Plant methods · 2016Article
- Microscale vortex-assisted electroporator for sequential molecular delivery.Journal of visualized experiments : JoVE · 2014Article
- Recent advances on host plants and expression cassettes' structure and function in plant molecular pharming.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2014Review
- MAPK transgenic circuit to improve plant stress-tolerance?Plant signaling & behavior · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At present, only few methods for the effective assembly of multigene constructs have been described. Here we present an improved version of the MultiRound Gateway technology, which facilitates plant multigene transformation. The system consists of two attL-flanked entry vectors, which contain an attR cassette, and a transformation-competent artificial chromosome based destination vector. By alternate use of the two entry vectors, multiple transgenes can be delivered sequentially into the Gateway-compatible destination vector. Multigene constructs that carried up to seven transgenes corresponding to more than 26 kb were assembled by seven rounds of LR recombination. The constructs were successfully transformed into tobacco plants and were stably inherited for at least two generations. Thus, our system represents a powerful, highly efficient tool for multigene plant transformation and may facilitate genetic engineering of agronomic traits or the assembly of genetic pathways for the production of biofuels, industrial or pharmaceutical compounds in plants.
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Registered trials
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