ArticleMicrobial biotechnology2012
Optimization of L-DOPA production by Brevundimonas sp. SGJ using response surface methodology.
Article in Microbial biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Foaming with Starch: Exploring Faba BeanFoods (Basel, Switzerland) · 2023Article
- Application of bacterial tyrosinases in organic synthesis.World journal of microbiology & biotechnology · 2021Review
- Pyomelanin-ProducingFrontiers in microbiology · 2021Article
- Response surface methodology: An effective optimization strategy for enhanced production of nitrile hydratase (NHase) byHeliyon · 2020Article
- Anti-Foc RT4 Activity of a Newly IsolatedFrontiers in microbiology · 2020Article
- Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of L-DOPA from D-glucose.Microbial cell factories · 2019Article
- Nutraceuticals in Parkinson's Disease.Neuromolecular medicine · 2016Review
- Genome engineering Escherichia coli for L-DOPA overproduction from glucose.Scientific reports · 2016Article
- Statistically optimized biotransformation protocol for continuous production of L-DOPA using Mucuna monosperma callus culture.SpringerPlus · 2013Article
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Authors and funding
4 authors.
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Abstract
L-DOPA (3,4-dihydroxyphenyl-L-alanine) is an extensively used drug for the treatment of Parkinson's disease. In the present study, optimization of nutritional parameters influencing L-DOPA production was attempted using the response surface methodology (RSM) from Brevundimonas sp. SGJ. A Plackett-Burman design was used for screening of critical components, while further optimization was carried out using the Box-Behnken design. The optimized levels of factors predicted by the model were pH 5.02, 1.549 g l(-1) tryptone, 4.207 g l(-1) L-tyrosine and 0.0369 g l(-1) CuSO(4) , which resulted in highest L-DOPA yield of 3.359 g l(-1). The optimization of medium using RSM resulted in a 8.355-fold increase in the yield of L-DOPA. The anova showed a significant R(2) value (0.9667), model F-value (29.068) and probability (0.001), with insignificant lack of fit. The highest tyrosinase activity observed was 2471 U mg(-1) at the 18th hour of the incubation period with dry cell weight of 0.711 g l(-1). L-DOPA production was confirmed by HPTLC, HPLC and GC-MS analysis. Thus, Brevundimonas sp. SGJ has the potential to be a new source for the production of L-DOPA.
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