ArticleStem cells and development2013
Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor.
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- Oxygen control in bioreactor drives high yield production of functional hiPSC-like hepatocytes for advanced liver disease modelling.Scientific reports · 2024Article
- Stem Cell-Based Strategies: The Future Direction of Bioartificial Liver Development.Stem cell reviews and reports · 2024Review
- Multicellular Liver Organoids: Generation and Importance of Diverse Specialized Cellular Components.Cells · 2023Review
- Article
- Current Status and Challenges of Human Induced Pluripotent Stem Cell-Derived Liver Models in Drug Discovery.Cells · 2022Review
- Physiologically relevant microsystems to study viral infection in the human liver.Frontiers in microbiology · 2022Review
- Utility of Common Marmoset (Genes · 2020Review
- Generation of qualified clinical-grade functional hepatocytes from human embryonic stem cells in chemically defined conditions.Cell death & disease · 2019Article
- Differentiation of human-induced pluripotent stem cell under flow conditions to mature hepatocytes for liver tissue engineering.Journal of tissue engineering and regenerative medicine · 2018Article
- Hepatic differentiation of human iPSCs in different 3D models: A comparative study.International journal of molecular medicine · 2017Article
- Scalable Differentiation of Human iPSCs in a Multicellular Spheroid-based 3D Culture into Hepatocyte-like Cells through Direct Wnt/β-catenin Pathway Inhibition.Scientific reports · 2016Article
- Cell sources for in vitro human liver cell culture models.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device.Stem cell research & therapy · 2016Article
- In vitro culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration.Protein & cell · 2015Review
- Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation.Journal of visualized experiments : JoVE · 2015Article
- Human pluripotent stem cell-derived products: advances towards robust, scalable and cost-effective manufacturing strategies.Biotechnology journal · 2015Review
- Engrafted human stem cell-derived hepatocytes establish an infectious HCV murine model.The Journal of clinical investigation · 2014Article
- Stem Cell Strategies to Evaluate Idiosyncratic Drug-induced Liver Injury.Journal of clinical and translational hepatology · 2014Review
- Cell and tissue engineering for liver disease.Science translational medicine · 2014Review
- Assembly of human organs from stem cells to study liver disease.The American journal of pathology · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drug-induced liver injury is a serious and frequently occurring adverse drug reaction in the clinics and is hard to predict during preclinical studies. Today, primary hepatocytes are the most frequently used cell model for drug discovery and prediction of toxicity. However, their use is marred by high donor variability regarding drug metabolism and toxicity, and instable expression levels of liver-specific genes such as cytochromes P450. An in vitro model system based on human embryonic stem cells (hESC), with their unique properties of pluripotency and self-renewal, has potential to provide a stable and unlimited supply of human hepatocytes. Much effort has been made to direct hESC toward the hepatic lineage, mostly using 2-dimensional (2D) cultures. Although the results are encouraging, these cells lack important functionality. Here, we investigate if hepatic differentiation of hESC can be improved by using a 3-dimensional (3D) bioreactor system. Human ESCs were differentiated toward the hepatic lineage using the same cells in either the 3D or 2D system. A global transcriptional analysis identified important differences between the 2 differentiation regimes, and we identified 10 pathways, highly related to liver functions, which were significantly upregulated in cells differentiated in the bioreactor compared to 2D control cultures. The enhanced hepatic differentiation observed in the bioreactor system was also supported by immunocytochemistry. Taken together, our results suggest that hepatic differentiation of hESC is improved when using this 3D bioreactor technology as compared to 2D culture systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.