ArticlePloS one2012
The effect of environmental conditions on biofilm formation of Burkholderia pseudomallei clinical isolates.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 69 citations in OpenAlex.
- Colony morphotype variation inJournal of bacteriology · 2025Review
- A High-Throughput Microtiter Plate Screening Assay to Quantify and Differentiate Species in Dual-Species Biofilms.Microorganisms · 2023Article
- Biofilm Signaling, Composition and Regulation inJournal of microbiology and biotechnology · 2023Review
- Genomic comparison and phenotypic profiling of small colony variants of Burkholderia pseudomallei.PloS one · 2021Article
- Hijacking of the Host's Immune Surveillance Radars byFrontiers in immunology · 2021Review
- Biofilm Formation, Production of Matrix Compounds and Biosorption of Copper, Nickel and Lead by Different Bacterial Strains.Frontiers in microbiology · 2021Article
- Influence of Selected Factors on Biofilm Formation byMicroorganisms · 2020Article
- ScmR, a Global Regulator of Gene Expression, Quorum Sensing, pH Homeostasis, and Virulence inJournal of bacteriology · 2020Article
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- Evaluating the role of Burkholderia pseudomallei K96243 toxins BPSS0390, BPSS0395, and BPSS1584 in persistent infection.Cellular microbiology · 2019Article
- Burkholderia pseudomallei-absent soil bacterial community results in secondary metabolites that kill this pathogen.AMB Express · 2018Article
- Melioidosis in Malaysia: Incidence, Clinical Challenges, and Advances in Understanding Pathogenesis.Tropical medicine and infectious disease · 2018Review
- Secondary metabolites from Bacillus amyloliquefaciens isolated from soil can kill Burkholderia pseudomallei.AMB Express · 2017Article
- Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis.Scientific reports · 2017Article
- Persistent infection due to a small-colony variant of Burkholderia pseudomallei leads to PD-1 upregulation on circulating immune cells and mononuclear infiltration in viscera of experimental BALB/c mice.PLoS neglected tropical diseases · 2017Article
- Resource abundance and the critical transition to cooperation.Journal of evolutionary biology · 2017Article
- Thermoregulation of Biofilm Formation in Burkholderia pseudomallei Is Disrupted by Mutation of a Putative Diguanylate Cyclase.Journal of bacteriology · 2017Article
- Trehalase plays a role in macrophage colonization and virulence of Burkholderia pseudomallei in insect and mammalian hosts.Virulence · 2017Article
- Global gene expression inGut pathogens · 2017Article
- Impact of Nutrient Restriction on the Structure ofFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia pseudomallei, a Gram-negative saprophytic bacterium, is the causative agent of the potentially fatal melioidosis disease in humans. In this study, environmental parameters including temperature, nutrient content, pH and the presence of glucose were shown to play a role in in vitro biofilm formation by 28 B. pseudomallei clinical isolates, including four isolates with large colony variants (LCVs) and small colony variants (SCVs) morphotypes. Enhanced biofilm formation was observed when the isolates were tested in LB medium, at 30 °C, at pH 7.2, and in the presence of as little as 2 mM glucose respectively. It was also shown that all SVCs displayed significantly greater capacity to form biofilms than the corresponding LCVs when cultured in LB at 37 °C. In addition, octanoyl-homoserine lactone (C(8)-HSL), a quorum sensing molecule, was identified by mass spectrometry analysis in bacterial isolates referred to as LCV CTH, LCV VIT, SCV TOM, SCV CTH, 1 and 3, and the presence of other AHL's with higher masses; decanoyl-homoserine lactone (C(10)-HSL) and dodecanoyl-homoserine lactone (C(12)-HSL) were also found in all tested strain in this study. Last but not least, we had successfully acquired two Bacillus sp. soil isolates, termed KW and SA respectively, which possessed strong AHLs degradation activity. Biofilm formation of B. pseudomallei isolates was significantly decreased after treated with culture supernatants of KW and SA strains, demonstrating that AHLs may play a role in B. pseudomallei biofilm formation.
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Registered trials
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