ArticlePloS one2012
Mapping the mechanome of live stem cells using a novel method to measure local strain fields in situ at the fluid-cell interface.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
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- Influence of Magneto-Mechanical Stress on Cellular Membranes, Ion Channel Activity, and Gene Expression Dynamics.Results and problems in cell differentiation · 2026Review
- Biomechanical research using advanced micro-nano devices: In-Vitro cell Characterization focus.Journal of advanced research · 2025Review
- Stem cell mechanoadaptation. II. Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling.APL bioengineering · 2025Article
- Stem cell mechanoadaptation. I. Effect of microtubule stabilization and volume changing stresses on cytoskeletal remodeling.APL bioengineering · 2025Article
- Article
- Extended view on the mechanobiology of fracture healing: interplay between mechanics and inflammation.Frontiers in bioengineering and biotechnology · 2025Review
- The extracellular matrix mechanics in the vasculature.Nature cardiovascular research · 2023Review
- Biomechanical, biophysical and biochemical modulators of cytoskeletal remodelling and emergent stem cell lineage commitment.Communications biology · 2023Review
- Harnessing mechanical cues in the cellular microenvironment for bone regeneration.Frontiers in physiology · 2023Review
- Microengineered filters for efficient delivery of nanomaterials into mammalian cells.Scientific reports · 2022Article
- Shear-stress sensing by PIEZO1 regulates tendon stiffness in rodents and influences jumping performance in humans.Nature biomedical engineering · 2021Article
- Review
- Displacement and strain mapping for osteocytes under fluid shear stress using digital holographic microscopy and digital image correlation.Biomedical optics express · 2021Article
- Low intermittent flow promotes rat mesenchymal stem cell differentiation in logarithmic fluid shear device.Biomicrofluidics · 2020Article
- Mechanical Characterization for Cellular Mechanobiology: Current Trends and Future Prospects.Frontiers in bioengineering and biotechnology · 2020Review
- Mapping the Mechanome-A Protocol for Simultaneous Live Imaging and Quantitative Analysis of Cell Mechanoadaptation and Ingression.Bio-protocol · 2019Article
- Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation.Stem cell research & therapy · 2019Article
- Mechanomics Approaches to Understand Cell Behavior in Context of Tissue Neogenesis, During Prenatal Development and Postnatal Healing.Frontiers in cell and developmental biology · 2019Review
- Twenty years of 'insanity' in diagnosing underlying clinically relevant cervical dysfunction using traditional MRI.Journal of spine surgery (Hong Kong) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
During mesenchymal condensation, the initial step of skeletogenesis, transduction of minute mechanical forces to the nucleus is associated with up or down-regulation of genes, ultimately resulting in formation of the skeletal template and appropriate cell lineage commitment. The summation of these biophysical cues affects the cell's shape and fate. Here, we predict and measure surface strain, in live stem cells, in response to controlled delivery of stresses, providing a platform to direct short-term structure--function relationships and long-term fate decisions. We measure local strains on stem cell surfaces using fluorescent microbeads coated with Concanavalin A. During delivery of controlled mechanical stresses, 4-Dimensional (x,y,z,t) displacements of the bound beads are measured as surface strains using confocal microscopy and image reconstruction. Similarly, micro-particle image velocimetry (μ-piv) is used to track flow fields with fluorescent microspheres. The measured flow velocity gradient is used to calculate stress imparted by fluid drag at the surface of the cell. We compare strain measured on cell surfaces with those predicted computationally using parametric estimates of the cell's elastic and shear modulus. Finally, cross-correlating stress--strain data to measures of gene transcription marking lineage commitment enables us to create stress--strain--fate maps, for live stem cells in situ. The studies show significant correlations between live stem cell stress--strain relationships and lineage commitment. The method presented here provides a novel means to probe the live stem cell's mechanome, enabling mechanistic studies of the role of mechanics in lineage commitment as it unfolds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.