ArticleFrontiers in plant science2012
Using the Yeast Three-Hybrid System to Identify Proteins that Interact with a Phloem-Mobile mRNA.
Article in Frontiers in plant science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- PHR-Mediated Pi Starvation Response Mobile Messenger RNAs Represent Noncoding Transcripts in Recipient Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Screening and identification of photoresponse factors in kiwifruit (Actinidia arguta) development.Molecular biology reports · 2024Article
- BEL transcription factors in prominent Solanaceae crops: the missing pieces of the jigsaw in plant development.Planta · 2023Review
- RNA-Protein Interactome at the Hepatitis E Virus Internal Ribosome Entry Site.Microbiology spectrum · 2023Article
- The mRNA mobileome: challenges and opportunities for deciphering signals from the noise.The Plant cell · 2023Review
- Identification of host factors binding to dengue and Zika virus subgenomic RNA by efficient yeast three-hybrid screens of the human ORFeome.RNA biology · 2021Article
- Molecular characterization of Arabidopsis thaliana LSH1 and LSH2 genes.Genes & genomics · 2020Article
- Protein Interactomics by Two-Hybrid Methods.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Transcriptional analysis of phloem-associated cells of potato.BMC genomics · 2015Article
- Two RNA recognition motif-containing proteins are plant mitochondrial editing factors.Nucleic acids research · 2015Article
- Identification of phloem-mobile mRNA.Journal of plant research · 2015Review
- Possible role of the Nt-4/1 protein in macromolecular transport in vascular tissue.Plant signaling & behavior · 2013Article
- Phloem RNA-binding proteins as potential components of the long-distance RNA transport system.Frontiers in plant science · 2013Article
- A perspective on photoperiodic phloem-mobile signals that control development.Frontiers in plant science · 2013Article
- Phloem-mobile messenger RNAs and root development.Frontiers in plant science · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterografting and RNA transport experiments have demonstrated the long-distance mobility of StBEL5 RNA, its role in controlling tuber formation, and the function of the 503-nt 3' untranslated region (UTR) of the RNA in mediating transport. Because the 3' UTR of StBEL5 is a key element in regulating several aspects of RNA metabolism, a potato leaf cDNA library was screened using the 3' UTR of StBEL5 as bait in the yeast three-hybrid (Y3H) system to identify putative partner RNA-binding proteins (RBPs). From this screen, 116 positive cDNA clones were isolated based on nutrient selection, HIS3 activation, and lacZ induction and were sequenced and classified. Thirty-five proteins that were predicted to function in either RNA- or DNA-binding were selected from this pool. Seven were monitored for their expression profiles and further evaluated for their capacity to bind to the 3' UTR of StBEL5 using β-galactosidase assays in the Y3H system and RNA gel-shift assays. Among the final selections were two RBPs, a zinc finger protein, and one protein, StLSH10, from a family involved in light signaling. In this study, the Y3H system is presented as a valuable tool to screen and verify interactions between target RNAs and putative RBPs. These results can shed light on the dynamics and composition of plant RNA-protein complexes that function to regulate RNA metabolism.
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