ArticleSensors (Basel, Switzerland)2012
Multiplex immunoassay platforms based on shape-coded poly(ethylene glycol) hydrogel microparticles incorporating acrylic acid.
Article in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Shape-Encoded Hydrogel Sensor Particles Enable Multiplex Odorant Detection Through Deep-learning Classification.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Hydroxypropyl Cellulose Assembled Microspheres as Structural Color Barcodes from Revolving Microfluidics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Particle ID: A Multiplexed Hydrogel Bead Platform for Biomedical Applications.ACS applied materials & interfaces · 2023Article
- Single-Color Barcoding for Multiplexed Hydrogel Bead-Based Immunoassays.ACS applied materials & interfaces · 2022Article
- Recent Advances in Polymer Additive Engineering for Diagnostic and Therapeutic Hydrogels.International journal of molecular sciences · 2022Review
- Convection-driven microfabricated hydrogels for rapid biosensing.The Analyst · 2020Article
- Article
- Signal Improvement Strategies for Fluorescence Detection of Biomacromolecules.Journal of fluorescence · 2016Review
- Hydrogel microparticles for biosensing.European polymer journal · 2015Article
- Diverse applications of electronic-nose technologies in agriculture and forestry.Sensors (Basel, Switzerland) · 2013Review
- A versatile, bar-coded nuclear marker/reporter for live cell fluorescent and multiplexed high content imaging.PloS one · 2013Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A suspension protein microarray was developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles for potential applications in multiplex and high-throughput immunoassays. A simple photopatterning process produced various shapes of hydrogel micropatterns that were weakly bound to poly(dimethylsiloxane) (PDMS)-coated substrates. These micropatterns were easily detached from substrates during the washing process and were collected as non-spherical microparticles. Acrylic acids were incorporated into hydrogels, which could covalently immobilize proteins onto their surfaces due to the presence of carboxyl groups. The amount of immobilized protein increased with the amount of acrylic acid due to more available carboxyl groups. Saturation was reached at 25% v/v of acrylic acid. Immunoassays with IgG and IgM immobilized onto hydrogel microparticles were successfully performed with a linear concentration range from 0 to 500 ng/mL of anti-IgG and anti-IgM, respectively. Finally, a mixture of two different shapes of hydrogel microparticles immobilizing IgG (circle) and IgM (square) was prepared and it was demonstrated that simultaneous detection of two different target proteins was possible without cross-talk using same fluorescence indicator because each immunoassay was easily identified by the shapes of hydrogel microparticles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.