ArticleSensors (Basel, Switzerland)2012
Single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells.
Article in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- Electrolysis of Bacteria Based on Microfluidic Technology.Micromachines · 2023Article
- Review of Microfluidic Methods for Cellular Lysis.Micromachines · 2021Review
- Continuous electrical lysis of cancer cells in a microfluidic device with passivated interdigitated electrodes.Biomicrofluidics · 2020Article
- Rapid fabrication of sieved microwells and cross-flow microparticle trapping.Scientific reports · 2020Article
- Microfluidic single-cell analysis-Toward integration and total on-chip analysis.Biomicrofluidics · 2020Review
- Recent advances in microfluidic platforms for single-cell analysis in cancer biology, diagnosis and therapy.Trends in analytical chemistry : TRAC · 2019Article
- Localized Single-Cell Lysis and Manipulation Using Optothermally-Induced Bubbles.Micromachines · 2017Article
- Electrical lysis of cells for detergent-free droplet assays.Biomicrofluidics · 2016Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Digital Microfluidics for Manipulation and Analysis of a Single Cell.International journal of molecular sciences · 2015Review
- Shape-dependent optoelectronic cell lysis.Angewandte Chemie (International ed. in English) · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-μm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.