ArticleJournal of assisted reproduction and genetics2012
The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells.
Article in Journal of assisted reproduction and genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Oocyte-cumulus cell interaction: a key factor in early embryo development.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation.Scientific reports · 2024Article
- Metabolomics analysis of human cumulus cells obtained from cumulus-oocyte complexes with different developmental potential.Human reproduction (Oxford, England) · 2023Article
- Article
- Lipid Changes in the Peri-Implantation Period with Mass Spectrometry Imaging: A Systematic Review.Life (Basel, Switzerland) · 2023Review
- Follicular Fluid: A Powerful Tool for the Understanding and Diagnosis of Polycystic Ovary Syndrome.Biomedicines · 2022Review
- Article
- Lipid Identification and Transcriptional Analysis of Controlling Enzymes in Bovine Ovarian Follicle.International journal of molecular sciences · 2018Article
- Ovarian environment aging: follicular fluid lipidomic and related metabolic pathways.Journal of assisted reproduction and genetics · 2018Article
- Hyper response to ovarian stimulation affects the follicular fluid metabolomic profile of women undergoing IVF similarly to polycystic ovary syndrome.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Lipidomics analysis of follicular fluid by ESI-MS reveals potential biomarkers for ovarian endometriosis.Journal of assisted reproduction and genetics · 2015Article
- Article
- Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment.Journal of assisted reproduction and genetics · 2015Article
- Maldi-tof fingerprinting of seminal plasma lipids in the study of human male infertility.Lipids · 2014Article
- Hyaluronidase alters the lipid profile of cumulus cells as detected by MALDI-TOF MS and multivariate analysis.Lipids · 2014Article
- Fatty acid synthesis and oxidation in cumulus cells support oocyte maturation in bovine.Molecular endocrinology (Baltimore, Md.) · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThis research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy.
methodFifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built.
resultsS-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the non-pregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation.
conclusionWe conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs.
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