ArticleBMC microbiology2012
Novel screening assay for in vivo selection of Klebsiella pneumoniae genes promoting gastrointestinal colonisation.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- High burden of blaBMC microbiology · 2026Article
- Integrated oral-gut microbiota therapy: a novel perspective on preventing bacterial translocation for systemic disease management.Frontiers in cellular and infection microbiology · 2025Review
- Phenotypic and genomic changes in entericmSphere · 2024Article
- Glutamate production from aerial nitrogen using the nitrogen-fixing bacterium Klebsiella oxytoca.Communications biology · 2024Article
- Animal models ofFrontiers in microbiology · 2024Review
- Oral-Intestinal Microbiota in Colorectal Cancer: Inflammation and Immunosuppression.Journal of inflammation research · 2022Review
- Microbiota-mediated protection against antibiotic-resistant pathogens.Genes and immunity · 2021Review
- The Great ESKAPE: Exploring the Crossroads of Bile and Antibiotic Resistance in Bacterial Pathogens.Infection and immunity · 2020Review
- FNR-Dependent RmpA and RmpA2 Regulation of Capsule Polysaccharide Biosynthesis inFrontiers in microbiology · 2019Article
- Transcriptional regulation of bacterial virulence gene expression by molecular oxygen and nitric oxide.Virulence · 2014Review
- Article
- [Multiresistant gram-negative bacteria. A bacterial challenge of the twenty-first century].Medizinische Klinik, Intensivmedizin und Notfallmedizin · 2013Review
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundKlebsiella pneumoniae is an important opportunistic pathogen causing pneumonia, sepsis and urinary tract infections. Colonisation of the gastrointestinal (GI) tract is a key step in the development of infections; yet the specific factors important for K. pneumoniae to colonize and reside in the GI tract of the host are largely unknown. To identify K. pneumoniae genes promoting GI colonisation, a novel genomic-library-based approach was employed.
resultsScreening of a K. pneumoniae C3091 genomic library, expressed in E. coli strain EPI100, in a mouse model of GI colonisation led to the positive selection of five clones containing genes promoting persistent colonisation of the mouse GI tract. These included genes encoding the global response regulator ArcA; GalET of the galactose operon; and a cluster of two putative membrane-associated proteins of unknown function. Both ArcA and GalET are known to be involved in metabolic pathways in Klebsiella but may have additional biological actions beneficial to the pathogen. In support of this, GalET was found to confer decreased bile salt sensitivity to EPI100.
conclusionsThe present work establishes the use of genomic-library-based in vivo screening assays as a valuable tool for identification and characterization of virulence factors in K. pneumoniae and other bacterial pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.