ArticleAnalytical chemistry2012
A dual-column solid phase extraction strategy for online collection and preparation of continuously flowing effluent streams for mass spectrometry.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Recent advancements in sample pretreatment methods for glycoproteomics.Biophysics reports · 2026Article
- Integrated biosensors for monitoring microphysiological systems.Lab on a chip · 2022Review
- On-line pre-treatment, separation, and nanoelectrospray mass spectrometric determinations for pesticide metabolites and peptides based on a modular microfluidic platform.RSC advances · 2018Article
- Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry.Analytical and bioanalytical chemistry · 2017Article
- Real-time cellular exometabolome analysis with a microfluidic-mass spectrometry platform.PloS one · 2015Article
- Metabolic consequences of interleukin-6 challenge in developing neurons and astroglia.Journal of neuroinflammation · 2014Article
- The relevance and potential roles of microphysiological systems in biology and medicine.Experimental biology and medicine (Maywood, N.J.) · 2014Article
- Scaling and systems biology for integrating multiple organs-on-a-chip.Lab on a chip · 2013Review
- Engineering challenges for instrumenting and controlling integrated organ-on-chip systems.IEEE transactions on bio-medical engineering · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Current desalination techniques for mass spectrometry-based protocols are problematic for performing temporal response studies where increased temporal resolution requires small samples and faster sampling frequencies, which greatly increases the number of samples and sample preparation time. These challenges are pertinent to cellular dynamics experiments, where it is important to sample the biological system frequently and with as little sample waste as possible. To address these needs, we present a dual-column online solid phase extraction (SPE) approach capable of preconcentrating and preparing a constantly perfusing sample stream, with minimal to no sample loss. This strategy is evaluated for use in microfluidic bioreactor studies specifically aimed at characterizing suitable sample flow rates, temporal resolving power, and analyte concentrations. In this work, we demonstrate that this strategy may be used for flow rates as low as 500 nL/min, with temporal resolving power on the order of 3 min, with analyte loadings ranging from femtomoles to picomoles for metabolites. Under these conditions, recoveries of ca. 80% are obtained even at femtomole loadings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.