ArticleJournal of viral hepatitis2012
Mutations associated with occult hepatitis B virus infection result in decreased surface antigen expression in vitro.
Article in Journal of viral hepatitis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 54 citations in OpenAlex.
- Characterization of genetic mutations in hepatitis B virus isolated from HBsAg+/HBcAb+/HBsAb-/HBV DNA + Japanese blood donors.Scientific reports · 2025Article
- Identification and characteristics of mutations promoting occult HBV infection by ultrasensitive HBsAg assay.Journal of clinical microbiology · 2025Article
- Molecular Characterization of Hepatitis B Virus in People Living with HIV in Rural and Peri-Urban Communities in Botswana.Biomedicines · 2024Article
- Hepatitis B Virus Prevalence among HIV-Uninfected People Living in Rural and Peri-Urban Areas in Botswana.Microorganisms · 2024Article
- Incidence of Occult Hepatitis B Infection (OBI) and hepatitis B genotype characterization among blood donors in Cameroon.PloS one · 2024Article
- Low Risk of Occult Hepatitis B Infection among Vietnamese Blood Donors.Pathogens (Basel, Switzerland) · 2022Article
- Role of S protein transmembrane domain mutations in the development of occult hepatitis B virus infection.Emerging microbes & infections · 2022Article
- Hepatitis B Virus Research in South Africa.Viruses · 2022Review
- Molecular characteristics of the full-length genome of occult hepatitis B virus from blood donors in China.Scientific reports · 2022Article
- Alternative hepatitis B virus DNA confirmatory algorithm identified occult hepatitis B virus infection in Chinese blood donors with non-discriminatory nucleic acid testing.Blood transfusion = Trasfusione del sangue · 2022Article
- In silico analysis of mutations associated with occult hepatitis B virus (HBV) infection in South Africa.Archives of virology · 2021Article
- Evaluation of the in situ assay for HBV DNA: An observational real-world study in chronic hepatitis B.Medicine · 2021Observational
- Endemic HBV among hospital in-patients in Bangladesh, including evidence of occult infection.The Journal of general virology · 2021Article
- E2 Site Mutations in S Protein Strongly Affect Hepatitis B Surface Antigen Detection in the Occult Hepatitis B Virus.Frontiers in microbiology · 2021Article
- Hepatitis B Virus (HBV) Infection and Re-activation During Nucleos(t)ide Reverse Transcriptase Inhibitor-Sparing Antiretroviral Therapy in a High-HBV Endemicity Setting.Open forum infectious diseases · 2018Article
- Article
- In Silico Analysis of Hepatitis B Virus Occult Associated Mutations in Botswana Using a Novel Algorithm.Genes · 2018Article
- Hepatitis B Virus Blood Screening: Need for Reappraisal of Blood Safety Measures?Frontiers in medicine · 2018Review
- Characteristics of escape mutations from occult hepatitis B virus infected patients with hematological malignancies in South Egypt.World journal of hepatology · 2017Article
- HBsAg may reappear following reactivation in individuals with spontaneous HBsAg seroclearance 8 years previously.Epidemiology and infection · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Occult hepatitis B virus (HBV) infection is characterized by the absence of detectable hepatitis B surface antigen (HBsAg) in the serum, despite detectable HBV DNA. Investigations of the mechanisms underlying the development of occult HBV infection are lacking in the current literature, although viral mutations in the surface region, resulting in decreased HBsAg expression or secretion, represent one potential mechanism. Wild-type HBsAg expression vectors were constructed from genotype-matched chronic HBV sequences. Site-directed mutagenesis was then utilized to introduce three genotype A mutations - M103I, K122R and G145A - associated with occult HBV infection in vivo, alone and in combination, into the wild-type HBsAg vectors. Transfection of Huh7 and HepG2 cell lines was performed, and cell culture supernatants and cell lysates were collected over 7 days to assess the effects of these mutations on extracellular and intracellular HBsAg levels. The G145A mutation resulted in significantly decreased extracellular and intracellular HBsAg expression in vitro. The most pronounced reduction in HBsAg expression was observed when all three mutations were present. The mutations evaluated in vitro in the current study resulted in decreased HBsAg expression and potentially increased hepatic retention and/or decreased hepatic secretion of synthesized HBsAg, which could explain the lack of HBsAg detection that is characteristic of occult HBV infection in vivo.
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