ArticleJournal of the American Chemical Society2012
Density variant glycan microarray for evaluating cross-linking of mucin-like glycoconjugates by lectins.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 160 citations in OpenAlex.
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- Controlling the Sulfation Density of Glycosaminoglycan Glycopolymer Mimetics Enables High Antiviral Activity against SARS-CoV-2 and Reduces Anticoagulant Activity.Biomacromolecules · 2025Article
- Understanding Viral-Glycocalyx Interactions Using Brush-Linked to Cross-Linked Models.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Synthetic Mucins as Glycan-Defined Prebiotics.ACS central science · 2025Article
- Molecularly Defined Glycocalyx Models Reveal ABJACS Au · 2025Article
- Sub-10 μm Soft Interlayers Integrating Patterned Multivalent Biomolecular Binding Environments.ACS applied materials & interfaces · 2024Article
- Mannose Ligands for Mannose Receptor Targeting.International journal of molecular sciences · 2024Review
- The adaptive microbiome hypothesis and immune interactions in amphibian mucus.Developmental and comparative immunology · 2023Review
- Biocatalysis versus Molecular Recognition in Sialoside-Selective Neuraminidase Biosensing.ACS chemical biology · 2023Article
- Surface-Templated Glycopolymer Nanopatterns Transferred to Hydrogels for Designed Multivalent Carbohydrate-Lectin Interactions across Length Scales.Journal of the American Chemical Society · 2023Article
- Synthesis and biomedical applications of mucin mimic materials.Advanced drug delivery reviews · 2022Review
- A Useful Guide to Lectin Binding: Machine-Learning Directed Annotation of 57 Unique Lectin Specificities.ACS chemical biology · 2022Article
- Polymer-tethered glyconanoparticle colourimetric biosensors for lectin binding: structural and experimental parameters to ensure a robust output.RSC advances · 2022Article
- Glycopolymer-Based Materials: Synthesis, Properties, and Biosensing Applications.Topics in current chemistry (Cham) · 2022Review
- Principles of glycocalyx engineering with hydrophobic-anchored synthetic mucins.Frontiers in cell and developmental biology · 2022Article
- Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions.Frontiers in chemistry · 2022Article
- An Automated Approach to Assess Relative Galectin-Glycan Affinity Following Glycan Microarray Analysis.Frontiers in molecular biosciences · 2022Article
- Investigation of the Binding of Lectins with Polymer Glycoconjugates and the Glycoconjugates Containing Silver Nanoparticles by Means of Optical Spectroscopy and Light Scattering.Polymer science. Series A, Chemistry, physics · 2022Article
- Review
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Interactions of mucin glycoproteins with cognate receptors are dictated by the structures and spatial organization of glycans that decorate the mucin polypeptide backbone. The glycan-binding proteins, or lectins, that interact with mucins are often oligomeric receptors with multiple ligand binding domains. In this work, we employed a microarray platform comprising synthetic glycopolymers that emulate natural mucins arrayed at different surface densities to evaluate how glycan valency and spatial separation affect the preferential binding mode of a particular lectin. We evaluated a panel of four lectins (Soybean agglutinin (SBA), Wisteria floribunda lectin (WFL), Vicia villosa-B-4 agglutinin (VVA), and Helix pomatia agglutin (HPA)) with specificity for α-N-acetylgalactosamine (α-GalNAc), an epitope displayed on mucins overexpressed in many adenocarcinomas. While these lectins possess the ability to agglutinate A(1)-blood cells carrying the α-GalNAc epitope and cross-link low valency glycoconjugates, only SBA showed a tendency to form intermolecular cross-links among the arrayed polyvalent mucin mimetics. These results suggest that glycopolymer microarrays can reveal discrete higher-order binding preferences beyond the recognition of individual glycan epitopes. Our findings indicate that glycan valency can set thresholds for cross-linking by lectins. More broadly, well-defined synthetic glycopolymers enable the integration of glycoconjugate structural and spatial diversity in a single microarray screening platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.