ArticleReproductive biology and endocrinology : RB&E2012
Late morfofunctional alterations of the Sertoli cell caused by doxorubicin administered to prepubertal rats.
Article in Reproductive biology and endocrinology : RB&E, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 60 citations in OpenAlex.
- PRMT5 inhibition promotes cross-species spermatogonia expansion and suppresses differentiation.Cell regeneration (London, England) · 2026Article
- Abnormal Sperm Morphology in Lodpr/lncRNA-HSVIII Knockout Mice with Advancing Age.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- In vivo and in vitro spermatogenesis in prepubertal mouse testes exposed to low gonadotoxic doses of cytarabine or Daunorubicin.Scientific reports · 2025Article
- Non-alkylating agents-induced gonadotoxicity in pre-pubertal males: Insights on the clinical and pre-clinical front.Clinical and translational science · 2024Review
- Autophagy, a critical element in the aging male reproductive disorders and prostate cancer: a therapeutic point of view.Reproductive biology and endocrinology : RB&E · 2023Review
- Lovastatin promotes the self-renewal of murine and primate spermatogonial stem cells.Stem cell reports · 2023Article
- Inflammatory Modulation of miR-155 Inhibits Doxorubicin-Induced Testicular Dysfunction via SIRT1/FOXO1 Pathway: Insight into the Role of Acacetin and Bacillus cereus Protease.Applied biochemistry and biotechnology · 2022Article
- New insights into the activities and toxicities of the old anticancer drug doxorubicin.The FEBS journal · 2021Review
- Rat PRDM9 shapes recombination landscapes, duration of meiosis, gametogenesis, and age of fertility.BMC biology · 2021Article
- Chemotherapy induced damage to spermatogonial stem cells in prepubertal mouse in vitro impairs long-term spermatogenesis.Toxicology reports · 2021Article
- Protective effects ofInternational journal of reproductive biomedicine · 2020Article
- Exposure to Chemotherapy During Childhood or Adulthood and Consequences on Spermatogenesis and Male Fertility.International journal of molecular sciences · 2020Review
- Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague-Dawley rats.Cancer cell international · 2019Article
- How does chemotherapy treatment damage the prepubertal testis?Reproduction (Cambridge, England) · 2018Review
- Chemotherapy drugs cyclophosphamide, cisplatin and doxorubicin induce germ cell loss in an in vitro model of the prepubertal testis.Scientific reports · 2018Article
- Irinotecan metabolite SN38 results in germ cell loss in the testis but not in the ovary of prepubertal mice.Molecular human reproduction · 2016Article
- Effect of nano-zinc oxide on doxorubicin- induced oxidative stress and sperm disorders in adult male Wistar rats.Iranian journal of reproductive medicine · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDoxorubicin is a potent chemotherapeutic drug used against a variety of cancers. It acts through interaction with polymerases and topoisomerase II and free radical production. Doxorubicin activity is not specific to cancer cells and can also damage healthy cells, especially those undergoing rapid proliferation, such as spermatogonia. In previous studies our group showed that etoposide, another topoisomarese II poison, causes irreversible damage to Sertoli cells. Thus, the aim of this study was to address the effects of doxorubicin on Sertoli cell morphology and function and on the seminiferous epithelium cycle when administered to prepubertal rats.
methodsPrepubertal rats received the dose of 5 mg/Kg of doxorubicin, which was fractioned in two doses: 3 mg/Kg at 15dpp and 2 mg/Kg at 22 dpp. The testes were collected at 40, 64 and 127 dpp, fixed in Bouin's liquid and submitted to transferrin immunolabeling for Sertoli cell function analysis. Sertoli cell morphology and the frequency of the stages of the seminiferous epithelium cycle were analyzed in PAS + H-stained sections.
resultsThe rats treated with doxorubicin showed reduction of transferrin labeling in the seminiferous epithelium at 40 and 64 dpp, suggesting that Sertoli cell function is altered in these rats. All doxorubicin-treated rats showed sloughing and morphological alterations of Sertoli cells. The frequency of the stages of the seminiferous epithelium cycle was also affected in all doxorubicin-treated rats. CONCLUSIONS AND DISCUSSION: These data show that doxorubicin administration during prepuberty causes functional and morphological late damage to Sertoli cells; such damage is secondary to the germ cell primary injury and contributed to enhance the spermatogenic harm caused by this drug. However, additional studies are required to clarify if there is also a direct effect of doxorubicin on Sertoli cells producing a primary damage on these cells.
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