ArticleThe Plant journal : for cell and molecular biology2013
Identification of an additional protein involved in mannan biosynthesis.
Article in The Plant journal : for cell and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 60 citations in OpenAlex.
- Co-expression of AkCSLA3 and AkMSR1 correlates with altered free monosaccharide pools in Pichia pastoris.BMC plant biology · 2026Article
- Atypical endo-β-1,4-mannanases are necessary for normal glucomannan synthesis in Arabidopsis.Plant physiology · 2026Article
- A characterization of recombinant Arabidopsis FRIABLE1 (FRB1) reveals robust rhamnogalacturonan-I rhamnosyltransferase activity and critical catalytic residues.The Journal of biological chemistry · 2026Article
- Unraveling mannan biosynthesis in poplar and spruce: functional characterization of Cellulose Synthase-Like family A (CSLA) genes.The New phytologist · 2026Article
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- A Fresh Look at Celery Collenchyma and Parenchyma Cell Walls Through a Combination of Biochemical, Histochemical, and Transcriptomic Analyses.International journal of molecular sciences · 2025Article
- Convergent Emergence of Glucomannan β-Galactosyltransferase Activity in Asterids and Rosids.Plant & cell physiology · 2024Article
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- Cytochemical localization and synthesis mechanism of the glucomannan in pseudobulbs ofHorticulture research · 2024Article
- Exploring the putative microRNAs cross-kingdom transfer inFrontiers in plant science · 2024Article
- Disruption of pollen tube homogalacturonan synthesis relieves pollen tube penetration defects in the Arabidopsis O-FUCOSYLTRANSFERASE1 mutant.Plant reproduction · 2023Article
- Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth inPlants (Basel, Switzerland) · 2023Article
- Glucomannan inGenes · 2022Review
- Transcriptomics Reveals Host-Dependent Differences of Polysaccharides Biosynthesis inMolecules (Basel, Switzerland) · 2021Article
- Mutation of an Arabidopsis Golgi membrane protein ELMO1 reduces cell adhesion.Development (Cambridge, England) · 2021Article
- Arabinogalactan Proteins in Plant Roots - An Update on Possible Functions.Frontiers in plant science · 2021Review
- Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development.BMC genomics · 2019Article
- Mechanistic insights from plant heteromannan synthesis in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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5 authors at 3 institutions in 2 countries.
Funding
Abstract
Galactomannans comprise a β-1,4-mannan backbone substituted with α-1,6-galactosyl residues. Genes encoding the enzymes that are primarily responsible for backbone synthesis and side-chain addition of galactomannans were previously identified and characterized. To identify additional genes involved in galactomannan biosynthesis, we previously performed deep EST profiling of fenugreek (Trigonella foenum-graecum L.) seed endosperm, which accumulates large quantities of galactomannans as a reserve carbohydrate during seed development. One of the candidate genes encodes a protein that is likely to be a glycosyltransferase. Because this protein is involved in mannan biosynthesis, we named it 'mannan synthesis-related' (MSR). Here, we report the characterization of a fenugreek MSR gene (TfMSR) and its two Arabidopsis homologs, AtMSR1 and AtMSR2. TfMSR was highly and specifically expressed in the endosperm. TfMSR, AtMSR1 and AtMSR2 proteins were all determined to be localized to the Golgi by fluorescence confocal microscopy. The level of mannosyl residues in stem glucomannans decreased by approximately 40% for Arabidopsis msr1 single T-DNA insertion mutants and by more than 50% for msr1 msr2 double mutants, but remained unchanged for msr2 single mutants. In addition, in vitro mannan synthase activity from the stems of msr1 single and msr1 msr2 double mutants also decreased. Expression of AtMSR1 or AtMSR2 in the msr1 msr2 double mutant completely or partially restored mannosyl levels. From these results, we conclude that the MSR protein is important for mannan biosynthesis, and offer some ideas about its role.
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