ArticleProceedings of the National Academy of Sciences of the United States of America1990
Thyroid hormone regulates expression of a transfected human alpha-myosin heavy-chain fusion gene in fetal rat heart cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 147 citations in OpenAlex.
- Elucidating the cellular determinants of the end-systolic pressure-volume relationship of the heart via computational modelling.The Journal of physiology · 2026Article
- Triad of palpitation, angina, and murmur in a non-cardiac patient.Journal of family medicine and primary care · 2021Article
- Comparison of myocardial deformation indices during rest and after activity in untreated hyperthyroid patients with normal population.American journal of cardiovascular disease · 2020Article
- The Role of Thyroid Diseases and their Medications in Cardiovascular Disorders: A Review of the Literature.Current cardiology reviews · 2020Review
- Regulation of cardiac transcription by thyroid hormone and Med13.Journal of molecular and cellular cardiology · 2019Article
- SIRT1 enzymatically potentiates 1,25-dihydroxyvitamin DThe Journal of steroid biochemistry and molecular biology · 2017Article
- Analysis of differential secondary effects of novel rexinoids: select rexinoid X receptor ligands demonstrate differentiated side effect profiles.Pharmacology research & perspectives · 2015Article
- Role of Oxidative Stress in Thyroid Hormone-Induced Cardiomyocyte Hypertrophy and Associated Cardiac Dysfunction: An Undisclosed Story.Oxidative medicine and cellular longevity · 2015Review
- Article
- Hyperthyroidism and cardiovascular complications: a narrative review on the basis of pathophysiology.Archives of medical science : AMS · 2013Article
- Oxidative stress and heart failure in altered thyroid States.TheScientificWorldJournal · 2012Review
- Fatty acid metabolism and thyroid hormones.Current trends in endocrinology · 2012Article
- Analysis of hairless corepressor mutants to characterize molecular cooperation with the vitamin D receptor in promoting the mammalian hair cycle.Journal of cellular biochemistry · 2010Article
- Factors controlling cardiac myosin-isoform shift during hypertrophy and heart failure.Journal of molecular and cellular cardiology · 2007Review
- Cluster characterisation and temporal expression of porcine sarcomeric myosin heavy chain genes.Journal of muscle research and cell motility · 2003Article
- Rapid in vivo functional analysis of transgenes in mice using whole body imaging of luciferase expression.Transgenic research · 2001Article
- Protein kinase-A dependent phosphorylation of transcription enhancer factor-1 represses its DNA-binding activity but enhances its gene activation ability.Nucleic acids research · 2000Article
- Article
- Hyperthyroid heart disease.Clinical cardiology · 2000Review
- Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1.The Journal of clinical investigation · 2000Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The rat alpha-myosin heavy-chain (alpha-MHC) gene is regulated by 3,5,3'-triiodo-L-thyronine (T3) in ventricular myocardium and is constitutively expressed in atrial tissue. Less is known about regulation of the human gene, but conservation of sequences in the 5'-flanking region between the rat and human alpha-MHC genes suggests that the human gene may be regulated similarly. Accordingly, T3-responsiveness and tissue-specific expression of human and rat alpha-MHC/chloramphenicol acetyltransferase fusion constructs have been compared in rat fetal heart cells, L6E9 myoblasts and myotubes, 3T3 fibroblasts, and HeLa cells. Transient transfection assays revealed a complex series of cis-regulatory elements in the 5'-flanking sequences in the human genes, including a basal promoter element with canonical TATAA and CAAT sequences, two positive regulatory element(s), and two negative regulatory elements, which markedly diminished both constitutive and T3-inducible activity. Interestingly, the human gene seemed to contain a proximal thyroid-hormone response element(s) not found in the rat gene. In L6E9 myoblasts and myotubes, the human constructs were constitutively expressed but not T3-regulated; none of the constructs were active in 3T3 or HeLa cells. We propose that interactions among the thyroid hormone responsive elements and other cis-acting elements in the human alpha-MHC 5'-flanking sequences may be sufficient to explain the characteristic features of expression of this gene in cardiac tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.