ArticleThe Journal of cell biology2012
Cell confinement controls centrosome positioning and lumen initiation during epithelial morphogenesis.
Article in The Journal of cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 102 citations in OpenAlex.
- Centrosome migration and apical membrane formation during epithelial polarization in MDCK cysts.eLife · 2026Article
- Promoting Polarization and Differentiation of Primary Human Salivary Gland Stem/Progenitor Cells in Protease-Degradable Hydrogels via ROCK Inhibition.ACS applied materials & interfaces · 2025Article
- Identification and validation of biomarkers related to centrosome replication in ulcerative colitis based on bulk transcriptome, single-cell RNA sequencing and experiments.Frontiers in immunology · 2025Article
- Morphogenetic Roles of Hydrostatic Pressure in Animal Development.Annual review of cell and developmental biology · 2022Review
- Intrinsic cell rheology drives junction maturation.Nature communications · 2022Article
- Primary URECs: a source to better understand the pathology of renal tubular epithelia in pediatric hereditary cystic kidney diseases.Orphanet journal of rare diseases · 2022Article
- Self-Organogenesis from 2D Micropatterns to 3D Biomimetic Biliary Trees.Bioengineering (Basel, Switzerland) · 2021Article
- Quantitative developmental biology in vitro using micropatterning.Development (Cambridge, England) · 2021Review
- Asynchrony of Apical Polarization, Luminogenesis, and Functional Differentiation in the Developing Thyroid Gland.Frontiers in endocrinology · 2021Article
- Biomimetic niches reveal the minimal cues to trigger apical lumen formation in single hepatocytes.Nature materials · 2020Article
- Apical poles without neighbouring cells.Nature materials · 2020Article
- Fibrocystin Is Essential to Cellular Control of Adhesion and Epithelial Morphogenesis.International journal of molecular sciences · 2020Article
- IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules.Nature communications · 2020Article
- Centering and Shifting of Centrosomes in Cells.Cells · 2020Review
- Application and Comparison of Supervised Learning Strategies to Classify Polarity of Epithelial Cell Spheroids in 3D Culture.Frontiers in genetics · 2020Article
- Myosin II isoforms play distinct roles ineLife · 2019Article
- Radial Profile Analysis of Epithelial Polarity in Breast Acini: A Tool for Primary (Breast) Cancer Prevention.Frontiers in medicine · 2019Article
- Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated αFrontiers in cell and developmental biology · 2019Article
- Cell polarity: having and making sense of direction-on the evolutionary significance of the primary cilium/centrosome organ in Metazoa.Open biology · 2018Review
- Signaling Networks in Epithelial Tube Formation.Cold Spring Harbor perspectives in biology · 2017Review
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epithelial organ morphogenesis involves sequential acquisition of apicobasal polarity by epithelial cells and development of a functional lumen. In vivo, cells perceive signals from components of the extracellular matrix (ECM), such as laminin and collagens, as well as sense physical conditions, such as matrix stiffness and cell confinement. Alteration of the mechanical properties of the ECM has been shown to promote cell migration and invasion in cancer cells, but the effects on epithelial morphogenesis have not been characterized. We analyzed the effects of cell confinement on lumen morphogenesis using a novel, micropatterned, three-dimensional (3D) Madin-Darby canine kidney cell culture method. We show that cell confinement, by controlling cell spreading, limits peripheral actin contractility and promotes centrosome positioning and lumen initiation after the first cell division. In addition, peripheral actin contractility is mediated by master kinase Par-4/LKB1 via the RhoA-Rho kinase-myosin II pathway, and inhibition of this pathway restores lumen initiation in minimally confined cells. We conclude that cell confinement controls nuclear-centrosomal orientation and lumen initiation during 3D epithelial morphogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.