ArticleGenesis (New York, N.Y. : 2000)2013
Generation of a germ cell-specific mouse transgenic CHERRY reporter, Sohlh1-mCherryFlag.
Article in Genesis (New York, N.Y. : 2000), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 10 citations in OpenAlex.
- AAV9 Gene Therapy for Ovarian Dysfunction and Reproductive Aging: Molecular Mechanisms, Therapeutic Targets, and Translational Perspectives.International journal of molecular sciences · 2026Review
- Review
- The Factors and Pathways Regulating the Activation of Mammalian Primordial FolliclesFrontiers in cell and developmental biology · 2020Review
- Activation of Notch Signaling by Oocytes and Jag1 in Mouse Ovarian Granulosa Cells.Endocrinology · 2019Article
- Advanced immunostaining approaches to study early male germ cell development.Stem cell research · 2018Article
- DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis.Biology of reproduction · 2017Article
- Geography of follicle formation in the embryonic mouse ovary impacts activation pattern during the first wave of folliculogenesis.Biology of reproduction · 2015Article
- Identification of a β-galactosidase transgene that provides a live-cell marker of transcriptional activity in growing oocytes and embryos.Molecular human reproduction · 2015Article
- A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor.PloS one · 2014Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Visualization of differentiating germ cells is critical to understanding the formation of primordial follicles in the ovary, and the commitment of spermatogonial stem cells to differentiation. We engineered and generated a BAC transgenic mouse line, Sohlh1-mCherryFlag (S1CF), under the direction of the native Sohlh1 promoter. Sohlh1 is a germ cell-specific gene that encodes the basic helix-loop-helix (bHLH) transcriptional regulator that is essential in oogenesis and spermatogenesis. Sohlh1 expression is unique, and is limited to perinatal and early follicle oocytes and differentiating spermatogonia. The Sohlh1-mCherryFlag transgene was engineered to fuse SOHLH1 to the red fluorescent protein CHERRY with 3-tandem-FLAG tags. S1CF animals fluoresce specifically in the oocytes of perinatal ovaries and small follicles in adult ovaries, as well as in spermatogonia, a pattern that is similar to endogenous SOHLH1. Moreover, S1CF rescued germ cell loss and infertility in both male and female Sohlh1(-/-) animals. The FLAG-tag on S1CF was effective for immunostaining and immunoprecipitation. The Sohlh1-mCherryFlag transgenic mouse provides a unique model to study early germ cell differentiation, as well as in vivo imaging and purification of differentiating germ cells.
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