ArticleProceedings of the National Academy of Sciences of the United States of America1990
Involvement of myosin light-chain kinase in endothelial cell retraction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
66 citing papers in PubMed.
- Article
- Noncanonical function of long myosin light chain kinase in increasing ER-PM junctions and augmentation of SOCE.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Unleashing shear: Role of intercellular traction and cellular moments in collective cell migration.Biochemical and biophysical research communications · 2020Article
- Endothelial Activation: The Ang/Tie Axis in Sepsis.Frontiers in immunology · 2018Review
- Using a Novel Microfabricated Model of the Alveolar-Capillary Barrier to Investigate the Effect of Matrix Structure on Atelectrauma.Scientific reports · 2017Article
- The p38 pathway, a major pleiotropic cascade that transduces stress and metastatic signals in endothelial cells.Oncotarget · 2017Review
- Characterization of vascular permeability using a biomimetic microfluidic blood vessel model.Biomicrofluidics · 2017Article
- Mechanisms of modulation of brain microvascular endothelial cells function by thrombin.Brain research · 2017Article
- Rho, ROCK and actomyosin contractility in metastasis as drug targets.F1000Research · 2016Review
- Histamine activates p38 MAP kinase and alters local lamellipodia dynamics, reducing endothelial barrier integrity and eliciting central movement of actin fibers.American journal of physiology. Cell physiology · 2015Article
- Enhanced permeability responses to inflammation in streptozotocin-induced diabetic rat venules: Rho-mediated alterations of actin cytoskeleton and VE-cadherin.American journal of physiology. Heart and circulatory physiology · 2014Article
- Active contractions in single suspended epithelial cells.European biophysics journal : EBJ · 2014Article
- Proteomic changes between male and female worms of the polychaetous annelid Neanthes arenaceodentata before and after spawning.PloS one · 2013Article
- Sphingolipids in lung endothelial biology and regulation of vascular integrity.Handbook of experimental pharmacology · 2013Review
- Vascular remodeling alters adhesion protein and cytoskeleton reactions to inflammatory stimuli resulting in enhanced permeability increases in rat venules.Journal of applied physiology (Bethesda, Md. : 1985) · 2012Article
- Severely blunted allergen-induced pulmonary Th2 cell response and lung hyperresponsiveness in type 1 transient receptor potential channel-deficient mice.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- The Ca(2+) sensor stromal interaction molecule 1 (STIM1) is necessary and sufficient for the store-operated Ca(2+) entry function of transient receptor potential canonical (TRPC) 1 and 4 channels in endothelial cells.Molecular pharmacology · 2012Article
- Studies on the cell biology of interendothelial cell gaps.American journal of physiology. Lung cellular and molecular physiology · 2012Review
- Expression of calmodulin and myosin light chain kinase during larval settlement of the Barnacle Balanus amphitrite.PloS one · 2012Article
- Temporal and spatial correlation of platelet-activating factor-induced increases in endothelial [Ca²⁺]i, nitric oxide, and gap formation in intact venules.American journal of physiology. Heart and circulatory physiology · 2011Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Permeabilized bovine pulmonary artery endothelial cell monolayers were used to investigate the mechanism of endothelial cell retraction. Postconfluent endothelial cells permeabilized with saponin retracted upon exposure to ATP and Ca2+. Retraction was accompanied by thiophosphorylation of 19,000-Da myosin light chains when adenosine 5'-[gamma-[35S]thio]triphosphate was included in the medium. Both retraction and thiophosphorylation of myosin light chains exhibited a graded quantitative dependence on Ca2+. When permeabilized monolayers were extracted in buffer D containing 100 mM KCl and 30 mM MgCl2 for 30 min, the cells failed to retract upon exposure to ATP and Ca2+, and no thiophosphorylation of myosin light chains occurred. The ability both to retract and to thiophosphorylate myosin light chains was restored by the addition to the permeabilized, extracted cells of myosin light-chain kinase and calmodulin together but not by either alone. These studies indicate that endothelial cell retraction, as does smooth muscle contraction, depends on myosin light-chain kinase phosphorylation of myosin light chains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.