ArticleInternational journal of cancer2013
Optical molecular imaging detects changes in extracellular pH with the development of head and neck cancer.
Article in International journal of cancer, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Aiming the magic bullet: targeted delivery of imaging and therapeutic agents to solid tumors by pHLIP peptides.Frontiers in pharmacology · 2024Review
- In Vivo Distribution and Therapeutic Efficacy of Radioiodine-Labeled pH-Low Insertion Peptide Variant 3 in a Mouse Model of Breast Cancer.Molecular imaging · 2022Article
- In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer.Cancers · 2021Article
- Acid specific dark quencher QC1 pHLIP for multi-spectral optoacoustic diagnoses of breast cancer.Scientific reports · 2019Article
- Near-simultaneous quantification of glucose uptake, mitochondrial membrane potential, and vascular parameters in murine flank tumors using quantitative diffuse reflectance and fluorescence spectroscopy.Biomedical optics express · 2018Article
- In vivo quantitative imaging of tumor pH by nanosonophore assisted multispectral photoacoustic imaging.Nature communications · 2017Article
- Applications of pHLIP Technology for Cancer Imaging and Therapy.Trends in biotechnology · 2017Review
- Imaging the Tumor Microenvironment.Advances in experimental medicine and biology · 2017Review
- Comparative Study of Tumor Targeting and Biodistribution of pH (Low) Insertion Peptides (pHLIP(®) Peptides) Conjugated with Different Fluorescent Dyes.Molecular imaging and biology · 2016Article
- Advancing theranostics with tumor-targeting peptides for precision otolaryngology.World journal of otorhinolaryngology - head and neck surgery · 2016Review
- Contemporary use of sentinel lymph node biopsy in the head and neck.World journal of otorhinolaryngology - head and neck surgery · 2016Article
- Article
- The pH low insertion peptide pHLIP Variant 3 as a novel marker of acidic malignant lesions.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Residue-specific structures and membrane locations of pH-low insertion peptide by solid-state nuclear magnetic resonance.Nature communications · 2015Article
- Targeting acidity in diseased tissues: mechanism and applications of the membrane-inserting peptide, pHLIP.Archives of biochemistry and biophysics · 2015Article
- Widefield optical imaging of changes in uptake of glucose and tissue extracellular pH in head and neck cancer.Cancer prevention research (Philadelphia, Pa.) · 2014Article
- Targeting diseased tissues by pHLIP insertion at low cell surface pH.Frontiers in physiology · 2014Review
- Intraoperative imaging-guided cancer surgery: from current fluorescence molecular imaging methods to future multi-modality imaging technology.Theranostics · 2014Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Noninvasive localized measurement of extracellular pH in cancer tissues can have a significant impact on the management of cancer. Despite its significance, there are limited approaches for rapid and noninvasive measurement of local pH in a clinical environment. In this study, we demonstrate the potential of noninvasive topical delivery of Alexa-647 labeled pHLIP (pH responsive peptide conjugated with Alexa Fluor(®) 647) to image changes in extracellular pH associated with head and neck squamous cell carcinoma using widefield and high resolution imaging. We report a series of preclinical analyses to evaluate the optical contrast achieved after topical delivery of Alexa-647 labeled pHLIP in intact fresh human tissue specimens using widefield and high-resolution fluorescence imaging. Using topical delivery, Alexa-647 labeled pHLIP can be rapidly delivered throughout the epithelium of intact tissues with a depth exceeding 700 µm. Following labeling with Alexa-647 labeled pHLIP, the mean fluorescent contrast increased four to eight fold higher in clinically abnormal tissues as compared to paired clinically normal biopsies. Furthermore, the imaging approach showed significant differences in fluorescence contrast between the cancer and the normal biopsies across diverse patients and different anatomical sites (unpaired comparison). The fluorescence contrast differences between clinically abnormal and normal tissues were in agreement with the pathologic evaluation. Topical application of fluorescently labeled pHLIP can detect and differentiate normal from cancerous tissues using both widefield and high resolution imaging. This technology will provide an effective tool to assess tumor margins during surgery and improve detection and prognosis of head and neck cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.