ArticleMolecular biotechnology2013
First knockdown gene expression in bat (Hipposideros armiger) brain mediated by lentivirus.
Article in Molecular biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- Animal Models of Speech and Vocal Communication Deficits Associated With Psychiatric Disorders.Biological psychiatry · 2016Review
- A novel approach identifies the first transcriptome networks in bats: a new genetic model for vocal communication.BMC genomics · 2015Article
- Comparison of Lentiviral Packaging Mixes and Producer Cell Lines for RNAi Applications.Molecular biotechnology · 2015Article
- Insights into the genetic foundations of human communication.Neuropsychology review · 2015Review
- From the ultrasonic to the infrared: molecular evolution and the sensory biology of bats.Frontiers in physiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lentivirus-mediated RNA interference (RNAi) is a potent experimental tool for investigating gene functions in vitro and in vivo. It has advantages that transgenic technology lacks. However, in vivo applications are difficult to apply in the central nervous system of non-model organisms due to the lack of a standard brain atlas and genetic information. Here, we report the development of an in vivo gene delivery system used in bat brain tissue for the first time, based on lentivirus (LV) vectors expressing short hairpin RNA (shRNA) targeting Hipposideros armiger forkhead box P2 (FoxP2). In vitro transfection into HEK 293T cell with the vector bearing the cassettes encoding FoxP2 shRNA verified the knockdown efficiency. Pseudovirus particles were administered via stereotactic intracerebral microinjection into the anterior cingulate cortex of H. armiger. FoxP2 is of major interest because of its role in sensorimotor coordination and probably in echolocation. Subsequent in situ hybridization validated the in vivo silencing of the target gene. This report demonstrates that LV-mediated expression of RNAi could achieve effective gene silencing in bats, a non-model organism, and will assist in elucidating the functions of bat genes.
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Registered trials
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