ArticleNucleic acids research2012
Alkbh1 and Tzfp repress a non-repeat piRNA cluster in pachytene spermatocytes.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- IFI204 drives gasdermin D-mediated mitochondrial permeabilization to amplify neuronal pyroptosis in ischemic stroke.Journal of neuroinflammation · 2026Article
- ALKB-1-dependent tRNA methylation is required for efficient paternal mitochondrial elimination.Nature communications · 2026Article
- Of rodents and ruminants: a comparison of small noncoding RNA requirements in mouse and bovine reproduction.Journal of animal science · 2021Article
- Multi-substrate selectivity based on key loops and non-homologous domains: new insight into ALKBH family.Cellular and molecular life sciences : CMLS · 2021Review
- Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus.Parasites & vectors · 2016Article
- Tupaia small RNAs provide insights into function and evolution of RNAi-based transposon defense in mammals.RNA (New York, N.Y.) · 2015Article
- piRNAQuest: searching the piRNAome for silencers.BMC genomics · 2014Article
- Nucleic acid oxidation in DNA damage repair and epigenetics.Chemical reviews · 2014Review
- Epigenome-wide analysis of piRNAs in gene-specific DNA methylation.RNA biology · 2014Article
- Mammalian piRNAs: Biogenesis, function, and mysteries.Spermatogenesis · 2014Review
- The spliceosome as a transposon sensor.RNA biology · 2013Review
- Tzfp represses the androgen receptor in mouse testis.PloS one · 2013Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Piwi proteins and Piwi-interacting small RNAs (piRNAs) have known functions in transposon silencing in the male germline of fetal and newborn mice. Both are also present in adult testes; however, their function here remains a mystery. Here, we confirm that most piRNAs in meiotic spermatocytes originate from clusters in non-repeat intergenic regions of DNA. The regulation of these piRNA clusters, including the processing of the precursor transcripts into individual piRNAs, is accomplished through mostly unknown processes. We present a possible regulatory mechanism for one such cluster, named cluster 1082B, located on chromosome 7 in the mouse genome. The 1082B precursor transcript and its 788 unique piRNAs are repressed by the Alkbh1 dioxygenase and the testis-specific transcription repressor Tzfp. We observe a remarkable >1000-fold upregulation of individual piRNAs in pachytene spermatocytes isolated from Alkbh1- and Tzfp-deficient murine testes. Repression of cluster 1082B is further supported by the identification of a 10-bp Tzfp recognition sequence contained within the precursor transcript. Downregulation of LINE1 and IAP transcripts in the Alkbh1- and Tzfp-deficient mice leads us to propose a potential role for the 1082B-encoded piRNAs in transposon control.
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