ArticleEpigenetics2012
Measuring the methylome in clinical samples: improved processing of the Infinium Human Methylation450 BeadChip Array.
Article in Epigenetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed.
- Sex Differences in the Interaction of Epigenetic Risk and Trajectories of Default Mode Limbic Network Integration Predicting Childhood Anxiety.Research square · 2026Article
- Smoking-Associated DNA Methylation and Offspring Caries Experience: Findings from the GUSTO Study.Journal of dental research · 2026Article
- Maternal history of maltreatment interacts with DNA methylation patterns to predict infant temperament.Child abuse & neglect · 2025Article
- Genome-wide methylation and copy number variation analysis of medulloblastoma in a Saudi Arabian pediatric population.Annals of medicine and surgery (2012) · 2025Article
- Molecular signatures of invasive and non-invasive pituitary adenomas: a comprehensive analysis of DNA methylation and gene expression.BMC medicine · 2025Article
- Comparing methylation levels assayed in GC-rich regions with current and emerging methods.BMC genomics · 2024Article
- Identification of influential probe types in epigenetic predictions of human traits: implications for microarray design.Clinical epigenetics · 2022Article
- Dichotomy in the Impact of Elevated Maternal Glucose Levels on Neonatal Epigenome.The Journal of clinical endocrinology and metabolism · 2022Observational
- Influence of Maternal Lifestyle and Diet on Perinatal DNA Methylation Signatures Associated With Childhood Arterial Stiffness at 8 to 9 Years.Hypertension (Dallas, Tex. : 1979) · 2021Article
- Epigenetically dysregulated genes and pathways implicated in the pathogenesis of non-syndromic high myopia.Scientific reports · 2019Article
- Analysis of two birth tissues provides new insights into the epigenetic landscape of neonates born preterm.Clinical epigenetics · 2019Observational
- HOXA9 is a novel myopia risk gene.BMC ophthalmology · 2019Article
- In-utero epigenetic factors are associated with early-onset myopia in young children.PloS one · 2019Article
- Successful application of human-based methyl capture sequencing for methylome analysis in non-human primate models.BMC genomics · 2018Article
- Choice of surrogate tissue influences neonatal EWAS findings.BMC medicine · 2017Observational
- Genome-wide methylation analysis reveals differentially methylated loci that are associated with an age-dependent increase in bovine fibroblast response to LPS.BMC genomics · 2017Article
- DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- Circulating Tumor DNA as Biomarkers for Cancer Detection.Genomics, proteomics & bioinformatics · 2017Review
- Molecular Evidence for Differential Long-term Outcomes of Early Life Severe Acute Malnutrition.EBioMedicine · 2017Article
- Developmental pathways to adiposity begin before birth and are influenced by genotype, prenatal environment and epigenome.BMC medicine · 2017Article
Corrections and comments
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Authors and funding
18 authors.
Funding
Abstract
The Infinium Human Methylation450 BeadChip Array (TM) (Infinium 450K) is an important tool for studying epigenetic patterns associated with disease. This array offers a high-throughput, low cost alternative to more comprehensive sequencing-based methodologies. Here we compare data generated by interrogation of the same seven clinical samples by Infinium 450K and reduced representation bisulfite sequencing (RRBS). This is the largest data set comparing Infinium 450K array to the comprehensive RRBS methodology reported so far. We show good agreement between the two methodologies. A read depth of four or more reads in the RRBS data was sufficient to achieve good agreement with Infinium 450K. However, we observe that intermediate methylation values (20-80%) are more variable between technologies than values at the extremes of the bimodal methylation distribution. We describe careful processing of Infinium 450K data to correct for known limitations and batch effects. Using methodologies proposed by others and newly implemented and combined in this report, agreement of Infinium 450K data with independent techniques can be vastly improved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.