ArticleThe Journal of biological chemistry2012
Gene duplication and the evolution of hemoglobin isoform differentiation in birds.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 63 citations in OpenAlex.
- Origins of allostery in vertebrate hemoglobin evolution.bioRxiv : the preprint server for biology · 2026Article
- Convergent High OAnimals : an open access journal from MDPI · 2024Article
- Evolution and molecular basis of a novel allosteric property of crocodilian hemoglobin.Current biology : CB · 2023Article
- Changes in hemoglobin function and isoform expression during embryonic development in the American alligator,American journal of physiology. Regulatory, integrative and comparative physiology · 2021Article
- Changes in Hemoglobin Isoforms in the Peripheral Blood of Rats with Experimental Posthemorrhagic Anemia.Bulletin of experimental biology and medicine · 2021Article
- Recent genome duplications facilitate the phenotypic diversity of Hb repertoire in the Cyprinidae.Science China. Life sciences · 2021Article
- Genomic Insights into the Origin and Evolution of Molluscan Red-Bloodedness in the Blood Clam Tegillarca granosa.Molecular biology and evolution · 2021Article
- Evolved increases in hemoglobin-oxygen affinity and the Bohr effect coincided with the aquatic specialization of penguins.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Effect of NH2-terminal acetylation on the oxygenation properties of vertebrate haemoglobin.The Biochemical journal · 2020Article
- Biochemical pedomorphosis and genetic assimilation in the hypoxia adaptation of Tibetan antelope.Science advances · 2020Article
- Origin of complexity in haemoglobin evolution.Nature · 2020Article
- Structure and function of crocodilian hemoglobins and allosteric regulation by chloride, ATP, and COAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2020Article
- Oxygenation properties of hemoglobin and the evolutionary origins of isoform multiplicity in an amphibious air-breathing fish, the blue-spotted mudskipper (The Journal of experimental biology · 2020Article
- The role of mutation bias in adaptive molecular evolution: insights from convergent changes in protein function.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Allosteric mechanisms underlying the adaptive increase in hemoglobin-oxygen affinity of the bar-headed goose.The Journal of experimental biology · 2018Article
- Molecular basis of hemoglobin adaptation in the high-flying bar-headed goose.PLoS genetics · 2018Article
- Divergent and parallel routes of biochemical adaptation in high-altitude passerine birds from the Qinghai-Tibet Plateau.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Gene Turnover and Diversification of the α- and β-Globin Gene Families in Sauropsid Vertebrates.Genome biology and evolution · 2018Article
- Migration-Selection Balance Drives Genetic Differentiation in Genes Associated with High-Altitude Function in the Speckled Teal (Anas flavirostris) in the Andes.Genome biology and evolution · 2018Article
- Stability-Mediated Epistasis Restricts Accessible Mutational Pathways in the Functional Evolution of Avian Hemoglobin.Molecular biology and evolution · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The majority of bird species co-express two functionally distinct hemoglobin (Hb) isoforms in definitive erythrocytes as follows: HbA (the major adult Hb isoform, with α-chain subunits encoded by the α(A)-globin gene) and HbD (the minor adult Hb isoform, with α-chain subunits encoded by the α(D)-globin gene). The α(D)-globin gene originated via tandem duplication of an embryonic α-like globin gene in the stem lineage of tetrapod vertebrates, which suggests the possibility that functional differentiation between the HbA and HbD isoforms may be attributable to a retained ancestral character state in HbD that harkens back to a primordial, embryonic function. To investigate this possibility, we conducted a combined analysis of protein biochemistry and sequence evolution to characterize the structural and functional basis of Hb isoform differentiation in birds. Functional experiments involving purified HbA and HbD isoforms from 11 different bird species revealed that HbD is characterized by a consistently higher O(2) affinity in the presence of allosteric effectors such as organic phosphates and Cl(-) ions. In the case of both HbA and HbD, analyses of oxygenation properties under the two-state Monod-Wyman-Changeux allosteric model revealed that the pH dependence of Hb-O(2) affinity stems primarily from changes in the O(2) association constant of deoxy (T-state)-Hb. Ancestral sequence reconstructions revealed that the amino acid substitutions that distinguish the adult-expressed Hb isoforms are not attributable to the retention of an ancestral (pre-duplication) character state in the α(D)-globin gene that is shared with the embryonic α-like globin gene.
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