ArticleThe Journal of biological chemistry2012
Crystal structure of Arabidopsis Deg2 protein reveals an internal PDZ ligand locking the hexameric resting state.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- NAL1 forms a molecular cage to regulate FZP phase separation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- Chloroplasts Protein Quality Control and Turnover: A Multitude of Mechanisms.International journal of molecular sciences · 2022Review
- The Repeating, Modular Architecture of the HtrA Proteases.Biomolecules · 2022Article
- Chloroplast protease/chaperone AtDeg2 holds γPhotosynthetica · 2022Article
- Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility.Nature communications · 2020Article
- DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis.Journal of experimental botany · 2019Article
- Molecular motion regulates the activity of the Mitochondrial Serine Protease HtrA2.Cell death & disease · 2017Article
- Chloroplast Proteases: Updates on Proteolysis within and across Suborganellar Compartments.Plant physiology · 2016Review
- Insights into the Cyanobacterial Deg/HtrA Proteases.Frontiers in plant science · 2016Article
- Genome-wide analysis of adaptive molecular evolution in the carnivorous plant Utricularia gibba.Genome biology and evolution · 2015Article
- MET1 is a thylakoid-associated TPR protein involved in photosystem II supercomplex formation and repair in Arabidopsis.The Plant cell · 2015Article
- Regulation of ABCC6 trafficking and stability by a conserved C-terminal PDZ-like sequence.PloS one · 2014Article
- Characterization of diverse internal binding specificities of PDZ domains by yeast two-hybrid screening of a special peptide library.PloS one · 2014Article
- The structures of Arabidopsis Deg5 and Deg8 reveal new insights into HtrA proteases.Acta crystallographica. Section D, Biological crystallography · 2013Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic organelles have developed elaborate protein quality control systems to ensure their normal activity, among which Deg/HtrA proteases play an essential role. Plant Deg2 protease is a homologue of prokaryotic DegQ/DegP proteases and is located in the chloroplast stroma, where its proteolytic activity is required to maintain the efficiency of photosynthetic machinery during stress. Here, we demonstrate that Deg2 exhibits dual protease-chaperone activities, and we present the hexameric structure of Deg2 complexed with co-purified peptides. The structure shows that Deg2 contains a unique second PDZ domain (PDZ2) following a conventional PDZ domain (PDZ1), with PDZ2 orchestrating the cage assembly of Deg2. We discovered a conserved internal ligand for PDZ2 that mediates hexamer formation and thus locks the protease in the resting state. These findings provide insight into the diverse modes of PDZ domain-mediated regulation of Deg proteases.
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