ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2012
Insights from a novel model of slow-transit constipation generated by partial outlet obstruction in the murine large intestine.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Animal Models of Slow-Transit Constipation: Current Progress, Limitations, and Future Perspectives.Digestive diseases and sciences · 2026Review
- Brief Review: Rethinking Colonic Redundancy in Gastroenterology.Digestive diseases and sciences · 2026Review
- Burst-patterned stimulation restores colonic motility in preclinical models.Science translational medicine · 2025Article
- Deep learning for automatic volumetric bowel segmentation on body CT images.European radiology · 2025Article
- Enteric neuronal Piezo1 maintains mechanical and immunological homeostasis by sensing force.Cell · 2025Article
- Crosstalk between omega-6 oxylipins and the enteric nervous system: Implications for gut disorders?Frontiers in medicine · 2023Review
- The Shaggy Dog Story of Enteric Signaling: Serotonin, a Molecular Megillah.Advances in experimental medicine and biology · 2022Article
- Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways.Scientific reports · 2021Article
- Targeting Mechano-Transcription Process as Therapeutic Intervention in Gastrointestinal Disorders.Frontiers in pharmacology · 2021Review
- Activity within specific enteric neurochemical subtypes is correlated with distinct patterns of gastrointestinal motility in the murine colon.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Changes in murine anorectum signaling across the life course.Neurogastroenterology and motility · 2018Article
- A model of the enteric neural circuitry underlying the generation of rhythmic motor patterns in the colon: the role of serotonin.American journal of physiology. Gastrointestinal and liver physiology · 2017Review
- GABA-producing Bifidobacterium dentium modulates visceral sensitivity in the intestine.Neurogastroenterology and motility · 2017Article
- Irritable bowel syndrome evaluation using computed tomography colonography.World journal of gastroenterology · 2016Article
- The arachidonic acid metabolite 11β-ProstaglandinF2α controls intestinal epithelial healing: deficiency in patients with Crohn's disease.Scientific reports · 2016Article
- CrossTalk proposal: 5-HT is necessary for peristalsis.The Journal of physiology · 2015Article
- Use of Genetically Encoded Calcium Indicators (GECIs) Combined with Advanced Motion Tracking Techniques to Examine the Behavior of Neurons and Glia in the Enteric Nervous System of the Intact Murine Colon.Frontiers in cellular neuroscience · 2015Article
- Colonic migrating motor complexes, high amplitude propagating contractions, neural reflexes and the importance of neuronal and mucosal serotonin.Journal of neurogastroenterology and motility · 2014Article
- Important role of mucosal serotonin in colonic propulsion and peristaltic reflexes: in vitro analyses in mice lacking tryptophan hydroxylase 1.The Journal of physiology · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The mechanisms underlying slow-transit constipation (STC) are unclear. In 50% of patients with STC, some form of outlet obstruction has been reported; also an elongated colon has been linked to patients with STC. Our aims were 1) to develop a murine model of STC induced by partial outlet obstruction and 2) to determine whether this leads to colonic elongation and, consequently, activation of the inhibitory "occult reflex," which may contribute to STC in humans. Using a purse-string suture, we physically reduced the maximal anal sphincter opening in C57BL/6 mice. After 4 days, the mice were euthanized (acutely obstructed), the suture was removed (relieved), or the suture was removed and replaced repeatedly (chronically obstructed, over 24-31 days). In partially obstructed mice, we observed increased cyclooxygenase (COX)-2 levels in muscularis and mucosa, an elongated impacted large bowel, slowed transit, nonpropagating colonic migrating motor complexes (CMMCs), a lack of mucosal reflexes, a depolarized circular muscle with slow-wave activity due to a lack of spontaneous inhibitory junction potentials, muscle hypertrophy, and CMMCs in mucosa-free preparations. Elongation of the empty obstructed colon produced a pronounced occult reflex. Removal of the obstruction or addition of a COX-2 antagonist (in vitro and in vivo) restored membrane potential, spontaneous inhibitory junction potentials, CMMC propagation, and mucosal reflexes. We conclude that partial outlet obstruction increases COX-2 leading to a hyperexcitable colon. This hyperexcitability is largely due to suppression of only descending inhibitory nerve pathways by prostaglandins. The upregulation of motility is suppressed by the occult reflex activated by colonic elongation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.