Trial reportDiabetologia2012
Effects of short-term high-fat overfeeding on genome-wide DNA methylation in the skeletal muscle of healthy young men.
Trial report in Diabetologia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
109 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- DNA methylation alterations in muscle of critically ill patients.Journal of cachexia, sarcopenia and muscle · 2022Trial
- Effect of a diet containing folate and hazelnut oil capsule on the methylation level of theClinical epigenetics · 2017Trial
- Young men with low birthweight exhibit decreased plasticity of genome-wide muscle DNA methylation by high-fat overfeeding.Diabetologia · 2014Trial
- DNA methylation pattern in overweight women under an energy-restricted diet supplemented with fish oil.BioMed research international · 2014Trial
- Genome-Wide DNA Methylation Changes Induced by High-Fat Diet and Methyl Donor Supplementation in Female Lupus Models: An Exploratory Study.Molecular nutrition & food research · 2026Article
- Two Sides of a Coin: Molecular, metabolic, and Phenotypic Convergence in Pediatric Undernutrition and Obesity.Current obesity reports · 2026Review
- MethAgingDB: a comprehensive DNA methylation database for aging biology.Scientific data · 2025Article
- The role of MBD2 in immune cell development, function, and autoimmune diseases.Cell death discovery · 2025Review
- mInternational journal of molecular sciences · 2025Article
- Epigenetic Mechanisms of Obesity: Insights from Transgenic Animal Models.Life (Basel, Switzerland) · 2025Review
- Tissue-specific methylomic responses to a lifestyle intervention in older adults associate with metabolic and physiological health improvements.Aging cell · 2025Article
- Impact of excess sugar on the whole genome DNA methylation pattern in human sperm.Epigenomics · 2025Article
- The functional significance of vascular DNA hypermethylation in atherosclerosis: a historical perspective.Frontiers in pharmacology · 2025Review
- DNA methylation of exercise-responsive genes differs between trained and untrained men.BMC biology · 2024Article
- DNA methylation in human diseases.Heliyon · 2024Review
- DNA Methylation in the Adaptive Response to Exercise.Sports medicine (Auckland, N.Z.) · 2024Review
- Fetal programming effect of rumen-protected methionine on primiparous Angus × Simmental offspring's performance and skeletal muscle gene expression.Journal of animal science · 2024Article
- Role of epigenetic in cancer biology, in hematologic malignancies and in anticancer therapy.Frontiers in molecular medicine · 2024Review
- DNA Methylation of Birthweight-Blood Pressure Genes and Changes of Blood Pressure in Response to Weight-Loss Diets in the POUNDS Lost Trial.Hypertension (Dallas, Tex. : 1979) · 2023Article
49 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisEnergy-dense diets that are high in fat are associated with a risk of metabolic diseases. The underlying molecular mechanisms could involve epigenetics, as recent data show altered DNA methylation of putative type 2 diabetes candidate genes in response to high-fat diets. We examined the effect of a short-term high-fat overfeeding (HFO) diet on genome-wide DNA methylation patterns in human skeletal muscle.
methodsSkeletal muscle biopsies were obtained from 21 healthy young men after ingestion of a short-term HFO diet and a control diet, in a randomised crossover setting. DNA methylation was measured in 27,578 CpG sites/14,475 genes using Illumina's Infinium Bead Array. Candidate gene expression was determined by quantitative real-time PCR.
resultsHFO introduced widespread DNA methylation changes affecting 6,508 genes (45%), with a maximum methylation change of 13.0 percentage points. The HFO-induced methylation changes were only partly and non-significantly reversed after 6-8 weeks. Alterations in DNA methylation levels primarily affected genes involved in inflammation, the reproductive system and cancer. Few gene expression changes were observed and these had poor correlation to DNA methylation. CONCLUSIONS/
interpretationThe genome-wide DNA methylation changes induced by the short-term HFO diet could have implications for our understanding of transient epigenetic regulation in humans and its contribution to the development of metabolic diseases. The slow reversibility suggests a methylation build-up with HFO, which over time may influence gene expression levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.