ArticleBiosensors & bioelectronics2013
Automated processing integrated with a microflow cytometer for pathogen detection in clinical matrices.
Article in Biosensors & bioelectronics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Fabrication of spectroscopic microfluidic chips for mastitis detection in raw milk.Scientific reports · 2023Article
- Advances in automated real-time flow cytometry for monitoring of bioreactor processes.Engineering in life sciences · 2022Review
- Advances in microfluidic devices made from thermoplastics used in cell biology and analyses.Biomicrofluidics · 2017Review
- Evaluation of disposable microfluidic chip design for automated and fast Immunoassays.Biomicrofluidics · 2017Article
- Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection.Chemical Society reviews · 2015Review
- The intersection of flow cytometry with microfluidics and microfabrication.Lab on a chip · 2014Review
- A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors.Microbial cell factories · 2013Article
- Catch and release: integrated system for multiplexed detection of bacteria.Analytical chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A spinning magnetic trap (MagTrap) for automated sample processing was integrated with a microflow cytometer capable of simultaneously detecting multiple targets to provide an automated sample-to-answer diagnosis in 40 min. After target capture on fluorescently coded magnetic microspheres, the magnetic trap automatically concentrated the fluorescently coded microspheres, separated the captured target from the sample matrix, and exposed the bound target sequentially to biotinylated tracer molecules and streptavidin-labeled phycoerythrin. The concentrated microspheres were then hydrodynamically focused in a microflow cytometer capable of 4-color analysis (two wavelengths for microsphere identification, one for light scatter to discriminate single microspheres and one for phycoerythrin bound to the target). A three-fold decrease in sample preparation time and an improved detection limit, independent of target preconcentration, was demonstrated for detection of Escherichia coli 0157:H7 using the MagTrap as compared to manual processing. Simultaneous analysis of positive and negative controls, along with the assay reagents specific for the target, was used to obtain dose-response curves, demonstrating the potential for quantification of pathogen load in buffer and serum.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.