ArticleInvestigative ophthalmology & visual science2012
Effect of histamine on Ca(2+)-dependent signaling pathways in rat conjunctival goblet cells.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 28 citations in OpenAlex.
- Preclinical In Vitro Evaluation of an Innovative Nutraceutical Mixture Modulating the Histamine H1 Receptor-Preliminary Data.Current issues in molecular biology · 2026Article
- Pyrimidinergic P2Y1-Like Nucleotide Receptors Are Functional in Rat Conjunctival Goblet Cells.Investigative ophthalmology & visual science · 2025Article
- Article
- Resolvin D2 uses multiple CaJournal of cellular physiology · 2022Article
- Signaling Pathways Used by the Specialized Pro-Resolving Mediator Maresin 2 Regulate Goblet Cell Function: Comparison with Maresin 1.International journal of molecular sciences · 2022Article
- Functional role of histamine receptors in the renal cortical collecting duct cells.American journal of physiology. Cell physiology · 2022Article
- Resolvin D2 and Resolvin D1 Differentially Activate Protein Kinases to Counter-Regulate Histamine-Induced [CaInternational journal of molecular sciences · 2021Article
- Maresin 1, a specialized proresolving mediator, stimulates intracellular [CaJournal of cellular physiology · 2021Article
- Pro-Resolving Mediator Annexin A1 Regulates Intracellular CaFrontiers in immunology · 2021Article
- Anti-Allergic Potential of Cinnamaldehyde via the Inhibitory Effect of Histidine Decarboxylase (HDC) ProducingMolecules (Basel, Switzerland) · 2020Article
- Resolvin E1 Reduces Leukotriene B4-Induced Intracellular Calcium Increase and Mucin Secretion in Rat Conjunctival Goblet Cells.The American journal of pathology · 2020Article
- RvE1 uses the LTBThe ocular surface · 2020Article
- Resolvin D1, but not resolvin E1, transactivates the epidermal growth factor receptor to increase intracellular calcium and glycoconjugate secretion in rat and human conjunctival goblet cells.Experimental eye research · 2019Article
- Sodium channel TRPM4 and sodium/calcium exchangers (NCX) cooperate in the control of CaThe Journal of biological chemistry · 2019Article
- Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators.Scientific reports · 2018Article
- Signaling pathways activated by resolvin E1 to stimulate mucin secretion and increase intracellular CaExperimental eye research · 2018Article
- Activation of the EGF Receptor by Histamine Receptor Subtypes Stimulates Mucin Secretion in Conjunctival Goblet Cells.Investigative ophthalmology & visual science · 2018Article
- Lipoxin AMucosal immunology · 2017Article
- Neither non-toxigenicBMJ open ophthalmology · 2017Article
- Lipoxin AScientific reports · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe purpose of this study was to determine the Ca(2+)-dependent cellular signaling pathways used by histamine to stimulate conjunctival goblet cell secretion.
methodsCultured rat goblet cells were grown in RPMI 1640. Goblet cell secretion of high molecular weight glycoconjugates was measured by an enzyme-linked lectin assay. Intracellular [Ca(2+)] ([Ca(2+)](i)) was measured by loading cultured cells with the Ca(2+) sensitive dye fura-2. The level of [Ca(2+)](i) was measured using fluorescence microscopy. Extracellular regulated kinase (ERK) 2 was depleted using small interfering RNA (siRNA).
resultsHistamine-stimulated conjunctival goblet cell secretion of high molecular weight glycoproteins was blocked by removal of extracellular Ca(2+) and depletion of ERK2 by siRNA. Histamine increase in [Ca(2+)](i) was desensitized by repeated addition of agonist and blocked by a phospholipase C antagonist. Histamine at higher doses increased [Ca(2+)](i) by stimulating influx of extracellular Ca(2+), but at a lower dose released Ca(2+) from intracellular stores. Activation of each histamine receptor subtype (H(1)-H(4)) increased [Ca(2+)](i) and histamine stimulation was blocked by antagonists of each receptor subtype. The H(2) receptor subtype increase in [Ca(2+)](i) was cAMP dependent.
conclusionsWe conclude that histamine activates phospholipase C to release intracellular Ca(2+) that induces the influx of extracellular Ca(2+) and activates ERK1/2 to stimulate conjunctival goblet cell mucous secretion, and that activation of all four histamine receptor subtypes can increase [Ca(2+)](i).
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