ArticleMethods in molecular biology (Clifton, N.J.)2012
Maternal mRNA knock-down studies: antisense experiments using the host-transfer technique in Xenopus laevis and Xenopus tropicalis.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Oocyte Host-Transfer and Maternal mRNA Depletion Experiments inCold Spring Harbor protocols · 2018Article
- Gonadal Transcriptome Analysis of Pacific Abalone Haliotis discus discus: Identification of Genes Involved in Germ Cell Development.Marine biotechnology (New York, N.Y.) · 2018Article
- Role of maternal Xenopus syntabulin in germ plasm aggregation and primordial germ cell specification.Developmental biology · 2017Article
- High-efficiency non-mosaic CRISPR-mediated knock-in and indel mutation in F0Development (Cambridge, England) · 2017Article
- Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling.Developmental biology · 2017Review
- Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer.Developmental biology · 2017Article
- Controlling the Messenger: Regulated Translation of Maternal mRNAs in Xenopus laevis Development.Advances in experimental medicine and biology · 2017Review
- Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes.Development (Cambridge, England) · 2016Article
- A gradient of maternal Bicaudal-C controls vertebrate embryogenesis via translational repression of mRNAs encoding cell fate regulators.Development (Cambridge, England) · 2016Article
- Small C-terminal Domain Phosphatase 3 Dephosphorylates the Linker Sites of Receptor-regulated Smads (R-Smads) to Ensure Transforming Growth Factor β (TGFβ)-mediated Germ Layer Induction in Xenopus Embryos.The Journal of biological chemistry · 2015Article
- The dynamics of plus end polarization and microtubule assembly during Xenopus cortical rotation.Developmental biology · 2015Article
- Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis.Biology open · 2015Article
- Cas9-based genome editing in Xenopus tropicalis.Methods in enzymology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability to inhibit the activity of maternally stored gene products in Xenopus has led to numerous insights into early developmental mechanisms. Oocytes can be cultured and manipulated in vitro and then implanted into the body cavity of a host female to make them competent for fertilization. Here, we summarize the methods for obtaining, culturing, and fertilizing Xenopus oocytes, with the goal of inhibiting maternal gene function through antisense oligonucleotide-mediated mRNA knock-down. We describe a simplified technique for implanting donor oocytes into host females using intraperitoneal injection. Also, we present optimized methods for performing the host-transfer procedure with Xenopus tropicalis oocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.