ArticleIn vitro cellular & developmental biology. Animal2012
Effects of cell culture techniques on gene expression and cholesterol efflux in primary bovine mammary epithelial cells derived from milk and tissue.
Article in In vitro cellular & developmental biology. Animal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats.Food chemistry. Molecular sciences · 2025Article
- Current status and challenges for cell-cultured milk technology: a systematic review.Journal of animal science and biotechnology · 2024Review
- Investigation on the suitability of milk-derived primary bovine mammary epithelial cells grown on permeable membrane supports as an in vitro model for lactation.In vitro cellular & developmental biology. Animal · 2020Article
- Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk.In vitro cellular & developmental biology. Animal · 2017Article
- Relative gene expression of fatty acid synthesis genes at 60 days postpartum in bovine mammary epithelial cells of Surti and Jafarabadi buffaloes.Veterinary world · 2017Article
- Biological characterization of bovine mammary epithelial cell lines immortalized by HPV16 E6/E7 and SV40T.In vitro cellular & developmental biology. Animal · 2016Article
- AG and UAG induce β-casein expression via activation of ERK1/2 and AKT pathways.Journal of molecular endocrinology · 2016Article
- Deep Sequencing and Screening of Differentially Expressed MicroRNAs Related to Milk Fat Metabolism in Bovine Primary Mammary Epithelial Cells.International journal of molecular sciences · 2016Article
- Can widely used cell type markers predict the suitability of immortalized or primary mammary epithelial cell models?Biological research · 2016Article
- Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells.PloS one · 2016Article
- Mammary epithelial cells isolated from milk are a valuable, non-invasive source of mammary transcripts.Frontiers in genetics · 2015Review
- Lineage-specific markers of goat mammary cells in primary culture.In vitro cellular & developmental biology. Animal · 2014Article
- Cholesterol transport and regulation in the mammary gland.Journal of mammary gland biology and neoplasia · 2014Review
- Bovine mammary epithelial cell cultures for the study of mammary gland functions.In vitro cellular & developmental biology. Animal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primary bovine mammary epithelial cells (pbMEC) are often used in cell culture to study metabolic and inflammatory processes in the udder of dairy cows. The most common source is udder tissue from biopsy or after slaughter. However, it is also possible to culture them from milk, which is non-invasive, repeatable and yields less contamination with fibroblasts. Generally, not much is known about the influence of cell origin and cell culture techniques such as cryopreservation on pbMEC functionality. Cells were extracted from milk and udder tissue to evaluate if milk-derived pbMEC are a suitable alternative to tissue-derived pbMEC and to test what influence cryopreservation has. The cells were cultivated for three passages and stored in liquid nitrogen. The relative gene expression of the five target genes kappa-casein, lingual antimicrobial peptide (LAP), lactoferrin, lysozyme (LYZ1) and the prolactin receptor normalised with keratin 8 showed a tendency to decrease in the tissue cultures, but not in the milk-derived cultures, suggesting a greater influence of the cultivation process on tissue-derived cells, freezing lowered expression levels in both cultures. Overall expression of LAP and LYZ1 tended to be higher in milk cells. Cholesterol efflux was measured to compare passages one to seven in milk-derived cells. Passage number did not alter the efflux rate (p ≤ 0.05). We showed for the first time that the extraction of pbMEC from milk can be a suitable alternative to tissue extraction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.