ArticleNature protocols2012
Designing and using RNA scaffolds to assemble proteins in vivo.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review.Microbial cell factories · 2018Pooled it
- Synthetic biology tools to promote the folding and function of RNA aptamers in mammalian cells.RNA biology · 2023Article
- Application of artificial scaffold systems in microbial metabolic engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Metabolic pathway assembly using docking domains from type I cis-AT polyketide synthases.Nature communications · 2022Article
- Aptamer-Array-Guided Protein Assembly Enhances Synthetic mRNA Switch Performance.Angewandte Chemie (International ed. in English) · 2022Article
- Programmable allosteric DNA regulations for molecular networks and nanomachines.Science advances · 2022Article
- 2022 The 1st Western China symposium on the international frontier of synthetic biomanufacturing.Biotechnology notes (Amsterdam, Netherlands) · 2022Article
- RNA origami design tools enable cotranscriptional folding of kilobase-sized nanoscaffolds.Nature chemistry · 2021Article
- Synthetic Scaffold Systems for Increasing the Efficiency of Metabolic Pathways in Microorganisms.Biology · 2021Review
- Enzyme Assembly for Compartmentalized Metabolic Flux Control.Metabolites · 2020Review
- Modular enzyme assembly for enhanced cascade biocatalysis and metabolic flux.Nature communications · 2019Article
- Engineered synthetic scaffolds for organizing proteins within the bacterial cytoplasm.Nature chemical biology · 2018Article
- Composing RNA Nanostructures from a Syntax of RNA Structural Modules.Nano letters · 2017Article
- Mechanism of three-component collision to produce ultrastable pRNA three-way junction of Phi29 DNA-packaging motor by kinetic assessment.RNA (New York, N.Y.) · 2016Article
- Adding energy minimization strategy to peptide-design algorithm enables better search for RNA-binding peptides: Redesigned λ N peptide binds boxB RNA.Journal of computational chemistry · 2016Article
- U6atac snRNA stem-loop interacts with U12 p65 RNA binding protein and is functionally interchangeable with the U12 apical stem-loop III.Scientific reports · 2016Article
- Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities.Cold Spring Harbor perspectives in biology · 2016Review
- antaRNA: ant colony-based RNA sequence design.Bioinformatics (Oxford, England) · 2015Article
- Enzyme clustering accelerates processing of intermediates through metabolic channeling.Nature biotechnology · 2014Article
- In vivo co-localization of enzymes on RNA scaffolds increases metabolic production in a geometrically dependent manner.Nucleic acids research · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA scaffolds are synthetic noncoding RNA molecules with engineered 3D folding harnessed to spatially organize proteins in vivo. Here we provide a protocol to design, express and characterize RNA scaffolds and their cognate proteins within 1 month. The RNA scaffold designs described here are based on either monomeric or multimeric units harboring RNA aptamers as protein docking sites. The scaffolds and proteins are cloned into inducible plasmids and expressed to form functional assemblies. RNA scaffolds find applications in many fields in which in vivo organization of biomolecules is of interest. RNA scaffolds provide extended flexibility compared with DNA or protein scaffolding strategies through programmed modulation of multiple protein stoichiometry and numbers, as well as the proteins' relative distances and spatial orientations. For synthetic biology, RNA scaffolds provide a new platform that can be used to increase yields of sequential metabolic pathways.
Indexed as
Identifiers
22955695What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.