ArticleJournal of molecular biology2013
Structural insights into transcriptional repression by noncoding RNAs that bind to human Pol II.
Article in Journal of molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Reading the SINEs - functionality in noncoding RNA 'cheap genes'.RNA biology · 2026Review
- Structural insights into transcriptional regulation by the helicase RECQL5.Nature structural & molecular biology · 2025Article
- Mechanism of mammalian transcriptional repression by noncoding RNA.Nature structural & molecular biology · 2025Article
- Article
- Mechanism of herpesvirus protein kinase UL13 in immune escape and viral replication.Frontiers in immunology · 2022Review
- ALU non-B-DNA conformations, flipons, binary codes and evolution.Royal Society open science · 2020Review
- TDP-43 regulates transcription at protein-coding genes and Alu retrotransposons.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Article
- 7SK-BAF axis controls pervasive transcription at enhancers.Nature structural & molecular biology · 2016Article
- Article
- Repression of RNA Polymerase II Transcription by B2 RNA Depends on a Specific Pattern of Structural Regions in the RNA.Non-coding RNA · 2015Article
- Infection-Induced Retrotransposon-Derived Noncoding RNAs Enhance Herpesviral Gene Expression via the NF-κB Pathway.PLoS pathogens · 2015Article
- The non-coding B2 RNA binds to the DNA cleft and active-site region of RNA polymerase II.Journal of molecular biology · 2013Article
- Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5.Nature structural & molecular biology · 2013Article
- Struggling to let go: a non-coding RNA directs its own extension and destruction.The EMBO journal · 2013Article
- RNA polymerase II acts as an RNA-dependent RNA polymerase to extend and destabilize a non-coding RNA.The EMBO journal · 2013Article
- Three dimensional electron microscopy and in silico tools for macromolecular structure determination.EXCLI journal · 2013Review
- Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Gene transcription is regulated in response to environmental changes and developmental cues. In mammalian cells subjected to stress conditions such as heat shock, transcription of most protein-coding genes decreases, while the transcription of heat shock protein genes increases. Repression involves direct binding to RNA polymerase II (Pol II) of certain noncoding RNAs (ncRNAs) that are upregulated upon heat shock. Another class of ncRNAs is also upregulated and binds to Pol II but does not inhibit transcription. Incorporation of repressive ncRNAs into pre-initiation complexes prevents transcription initiation, while non-repressive ncRNAs are displaced from Pol II by TFIIF. Here, we present cryo-electron microscopy reconstructions of human Pol II in complex with six different ncRNAs from mouse and human. Our structures show that both repressive and non-repressive ncRNAs bind to a conserved binding site within the cleft of Pol II. The site, which is also shared with a previously characterized yeast aptamer, is close to the active center and, thus, in an ideal position to regulate transcription. Importantly, additional RNA elements extend flexibly beyond the docking site. We propose that the differences concerning the repressive activity of the ncRNAs analyzed must be due to the distinct character of these more unstructured, flexible segments of the RNA that emanate from the cleft.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.