Trial reportJournal of proteomics2012
A label-free proteome analysis strategy for identifying quantitative changes in erythrocyte membranes induced by red cell disorders.
Trial report in Journal of proteomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Comprehensive Human Red Blood Cell Membrane and Cytoplasmic Proteome Analyses.Scientific data · 2026Article
- Role of channels in the ObioRxiv : the preprint server for biology · 2025Article
- Measuring the effect of repetitive stretching on the deformability of human red blood cells using optical tweezers.Scientific reports · 2025Article
- RBC-GEM: A genome-scale metabolic model for systems biology of the human red blood cell.PLoS computational biology · 2025Article
- Proteomic Analysis of the Role of the Adenylyl Cyclase-cAMP Pathway in Red Blood Cell Mechanical Responses.Cells · 2022Article
- Article
- A novel essential splice site variant in SPTB in a large hereditary spherocytosis family.Molecular genetics & genomic medicine · 2021Article
- The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study.Metabolites · 2020Article
- Human erythrocytes: cytoskeleton and its origin.Cellular and molecular life sciences : CMLS · 2020Review
- Article
- Quantitative comparative analysis of human erythrocyte surface proteins between individuals from two genetically distinct populations.Communications biology · 2019Article
- Red blood cells are dynamic reservoirs of cytokines.Scientific reports · 2018Article
- A robust mass spectrometry method for rapid profiling of erythrocyte ghost membrane proteomes.Clinical proteomics · 2018Article
- CRISPR/Cas9 knockouts reveal genetic interaction between strain-transcendent erythrocyte determinants ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Multi-omics Evidence for Inheritance of Energy Pathways in Red Blood Cells.Molecular & cellular proteomics : MCP · 2016Article
- New insights on hereditary erythrocyte membrane defects.Haematologica · 2016Review
- Proteome analysis of the triton-insoluble erythrocyte membrane skeleton.Journal of proteomics · 2015Article
- Plasmodium vivax trophozoite-stage proteomes.Journal of proteomics · 2015Article
- Article
- Identification of secreted proteins that reflect autophagy dynamics within tumor cells.Autophagy · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Red blood cells have been extensively studied but many questions regarding membrane properties and pathophysiology remain unanswered. Proteome analysis of red cell membranes is complicated by a very wide dynamic range of protein concentrations as well as the presence of proteins that are very large, very hydrophobic, or heterogeneously glycosylated. This study investigated the removal of other blood cell types, red cell membrane extraction, differing degrees of fractionation using 1-D SDS gels, and label-free quantitative methods to determine optimized conditions for proteomic comparisons of clinical blood samples. The results showed that fractionation of red cell membranes on 1-D SDS gels was more efficient than low-ionic-strength extractions followed by 1-D gel fractionation. When gel lanes were sliced into 30 uniform slices, a good depth of analysis that included the identification of most well-characterized, low-abundance red cell membrane proteins including those present at 500 to 10,000 copies per cell was obtained. Furthermore, the size separation enabled detection of changes due to proteolysis or in vivo protein crosslinking. A combination of Rosetta Elucidator quantitation and subsequent statistical analysis enabled the robust detection of protein differences that could be used to address unresolved questions in red cell disorders. This article is part of a Special Issue entitled: Integrated omics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.