ArticlePLoS neglected tropical diseases2012
Detection of Giardia duodenalis assemblages A and B in human feces by simple, assemblage-specific PCR assays.
Article in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Comparison of multilocus genotyping and a commercial beta-giardin qPCR assay for detection of Giardia duodenalis zoonotic assemblages in cat and dog samples.Parasites & vectors · 2026Article
- High resolution melting real-time PCR for genotyping of Giardia lamblia assemblages A and B regardless of parasite load.Gut pathogens · 2025Article
- Molecular Investigation of Zoonotic Intestinal Protozoa in Pet Dogs and Cats in Yunnan Province, Southwestern China.Pathogens (Basel, Switzerland) · 2021Article
- Real-Time PCR for Molecular Detection of Zoonotic and Non-ZoonoticMicroorganisms · 2021Article
- The controversies surroundingCurrent research in parasitology & vector-borne diseases · 2021Review
- Molecular diversity of Giardia duodenalis in children under 5 years from the Manhiça district, Southern Mozambique enrolled in a matched case-control study on the aetiology of diarrhoea.PLoS neglected tropical diseases · 2021Article
- Molecular Characterization ofMicroorganisms · 2020Article
- Rapid on-site diagnosis of canine giardiosis: time versus performance.Parasites & vectors · 2020Article
- Role of Polymerase Chain Reaction in Stool and Duodenal Biopsy for Diagnosis of Giardiasis in Patients with Persistent/Chronic Diarrhea.Digestive diseases and sciences · 2020Article
- Genotyping of Giardia duodenalis in children in upper Egypt using assemblage- specific PCR technique.PloS one · 2020Article
- Prevalence ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019Article
- Comparative cost-effectiveness of immunoassays and FLOTAC for diagnosing Giardia spp. infection in dogs.Parasites & vectors · 2019Article
- Molecular characterisation and taxon assemblage typing of giardiasis in primary school children living close to the shoreline of Lake Albert, Uganda.Parasite epidemiology and control · 2019Article
- High occurrence of Blastocystis sp. subtypes 1-3 and Giardia intestinalis assemblage B among patients in Zanzibar, Tanzania.Parasites & vectors · 2016Article
- PCR-RFLP method to detect zoonotic and host-specific Giardia duodenalis assemblages in dog fecal samples.Parasitology research · 2016Article
- An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting.The Korean journal of parasitology · 2016Article
- Presence of zoonotic agents in engorged ticks and hedgehog faeces from Erinaceus europaeus in (sub) urban areas.Parasites & vectors · 2015Article
- A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A.BioMed research international · 2015Article
- Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba.Journal of parasitology research · 2015Article
- Genotyping of Giardia duodenalis isolates from dogs in Guangdong, China based on multi-locus sequence.The Korean journal of parasitology · 2014Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The flagellated protozoan Giardia duodenalis is a common gastrointestinal parasite of mammals, including humans. Molecular characterizations have shown the existence of eight genetic groups (or assemblages) in the G. duodenalis species complex. Human infections are caused by assemblages A and B, which infect other mammals as well. Whether transmission routes, animal reservoirs and associations with specific symptoms differ for assemblage A and assemblage B is not clear. Furthermore, the occurrence and clinical significance of mixed (A+B) infections is also poorly understood. To date, the majority of PCR assays has been developed to identify all G. duodenalis assemblages based on the use of primers that bind to conserved regions, yet a reliable identification of specific assemblages is better achieved by ad hoc methods. The aim of this work was to design simple PCR assays that, based on the use of assemblage-specific primers, produce diagnostic bands of different lengths for assemblage A and B. We first generated novel sequence information from assemblage B, identified homologous sequences in the assemblage A genome, and designed primers at six independent loci. Experiments performed on DNA extracted from axenic cultures showed that two of the six assays can detect the equivalent of a single cyst and are not negatively influenced by disproportions between DNA of each assemblage, at least up to a 9:1 ratio. Further experiments on DNAs extracted from feces showed that the two assays can detect both assemblages in single tube reactions with excellent reliability. Finally, the robustness of these assays was demonstrated by testing a large collection of human isolates previously typed by multi-locus genotyping.
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