ArticlePloS one2012
High resolution imaging of vascular function in zebrafish.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Integrated Metabolomics and Multilevel Validation of the Anti-Inflammatory and Skin-Soothing Effects of Bioactive Components fromInternational journal of molecular sciences · 2026Article
- Zebrafish model for studying vascular development and vascular-related disease.Frontiers in cell and developmental biology · 2026Review
- Multimodal Investigation of Angiogenesis and Its Prevention by Small Compounds in a Zebrafish Cancer Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Direct blood flow velocity measurements of zebrafish cerebral vessels with a dual-mode light-sheet microscope.Biomedical optics express · 2025Article
- Zebrafish as a Versatile Model for Cardiovascular Research: Peering into the Heart of the Matter.Cells · 2025Review
- Zebrafish fluorescence imaging platform based on Bessel light sheet illumination.Biomedical optics express · 2025Article
- Optical transparency and label-free vessel imaging of zebrafish larvae in shortwave infrared range as a tool for prolonged studying of cardiovascular system development.Scientific reports · 2022Article
- Article
- High-Throughput Imaging of Blood Flow Reveals Developmental Changes in Distribution Patterns of Hemodynamic Quantities in Developing Zebrafish.Frontiers in physiology · 2022Article
- Label-free quantitative measurement of cardiovascular dynamics in a zebrafish embryo using frequency-comb-referenced-quantitative phase imaging.Journal of biomedical optics · 2021Article
- Haemodynamic dependence of mechano-genetic evolution of the cardiovascular system in Japanese medaka.Journal of the Royal Society, Interface · 2021Article
- Blood Vessel Imaging at Pre-Larval Stages of Zebrafish Embryonic Development.Diagnostics (Basel, Switzerland) · 2020Article
- Functional optical coherence tomography and photoacoustic microscopy imaging for zebrafish larvae.Biomedical optics express · 2020Article
- Four-dimensional visualization of zebrafish cardiovascular and vessel dynamics by a structured illumination microscope with electrically tunable lens.Biomedical optics express · 2020Article
- Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish.Cell systems · 2019Article
- Fluid forces shape the embryonic heart: Insights from zebrafish.Current topics in developmental biology · 2019Review
- High-speed extended-volume blood flow measurement using engineered point-spread function.Biomedical optics express · 2018Article
- Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish.Nature communications · 2017Article
- Yap/Taz transcriptional activity is essential for vascular regression via Ctgf expression and actin polymerization.PloS one · 2017Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
rationaleThe role of the endothelium in the pathogenesis of cardiovascular disease is an emerging field of study, necessitating the development of appropriate model systems and methodologies to investigate the multifaceted nature of endothelial dysfunction including disturbed barrier function and impaired vascular reactivity.
objectiveWe aimed to develop and test an optimized high-speed imaging platform to obtain quantitative real-time measures of blood flow, vessel diameter and endothelial barrier function in order to assess vascular function in live vertebrate models. METHODS AND
resultsWe used a combination of cutting-edge optical imaging techniques, including high-speed, camera-based imaging (up to 1000 frames/second), and 3D confocal methods to collect real time metrics of vascular performance and assess the dynamic response to the thromboxane A(2) (TXA(2)) analogue, U-46619 (1 µM), in transgenic zebrafish larvae. Data obtained in 3 and 5 day post-fertilization larvae show that these methods are capable of imaging blood flow in a large (1 mm) segment of the vessel of interest over many cardiac cycles, with sufficient speed and sensitivity such that the trajectories of individual erythrocytes can be resolved in real time. Further, we are able to map changes in the three dimensional sizes of vessels and assess barrier function by visualizing the continuity of the endothelial layer combined with measurements of extravasation of fluorescent microspheres.
conclusionsWe propose that this system-based microscopic approach can be used to combine measures of physiologic function with molecular behavior in zebrafish models of human vascular disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.