ArticleInvestigative ophthalmology & visual science2012
CXCL1-deficient mice are highly sensitive to pseudomonas aeruginosa but not herpes simplex virus type 1 corneal infection.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Impact of innate immune factors on the persistence of Staphylococcus aureus in the ocular environment.Experimental eye research · 2025Article
- Recent advances in optical coherence tomography for anterior segment imaging in small animals and their clinical implications.The ocular surface · 2022Review
- The Importance of CXCL1 in Physiology and Noncancerous Diseases of Bone, Bone Marrow, Muscle and the Nervous System.International journal of molecular sciences · 2022Review
- Cytokines and chemokines: The vital role they play in herpes simplex virus mucosal immunology.Frontiers in immunology · 2022Review
- Foundational concepts in the biology of bacterial keratitis.Experimental eye research · 2021Review
- Pathogenesis of Herpes Stromal Keratitis: Immune Inflammatory Response Mediated by Inflammatory Regulators.Frontiers in immunology · 2020Review
- Ocular Glands Become Infected Secondarily to Infectious Keratitis and Play a Role in Corneal Resistance to Infection.Journal of virology · 2019Article
- Overexpression of MMPs in Corneas Requiring Penetrating and Deep Anterior Lamellar Keratoplasty.Investigative ophthalmology & visual science · 2019Article
- Rapamycin liposome gutta inhibiting fungal keratitis of rats.International journal of ophthalmology · 2019Article
- Fibroblast growth factor-2 drives and maintains progressive corneal neovascularization following HSV-1 infection.Mucosal immunology · 2018Article
- CXCL4 contributes to host defense against acute Pseudomonas aeruginosa lung infection.PloS one · 2018Article
- The Neonatal Fc Receptor and Complement Fixation Facilitate Prophylactic Vaccine-Mediated Humoral Protection against Viral Infection in the Ocular Mucosa.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- MyD88 contribution to ocular surface homeostasis.PloS one · 2017Article
- Therapeutic Use of Soluble Fas Ligand Ameliorates Acute and Recurrent Herpetic Stromal Keratitis in Mice.Investigative ophthalmology & visual science · 2015Article
- Epoxide-mediated differential packaging of Cif and other virulence factors into outer membrane vesicles.Journal of bacteriology · 2014Article
- HSV-1 targets lymphatic vessels in the eye and draining lymph node of mice leading to edema in the absence of a functional type I interferon response.The American journal of pathology · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine the role of the chemokine CXCL1 on leukocyte recruitment, cytokine production and host resistance during HSV-1 and Pseudomonas aeruginosa infection.
methodsViral titer and bacterial load were compared following infection of wild-type (WT) and CXCL1(-/-) mice. Corneal leukocyte recruitment was determined using flow cytometry. Cytokine levels were assessed by luminex-based suspension arrays. Hematoxylin and eosin (H&E) staining, confocal microscopy, and optical coherence tomography (OCT) were used to visualize leukocyte recruitment and corneal thickening.
resultsHSV-1-infected WT and CXCL1(-/-) mice possessed similar viral titers in the cornea during late acute infection. Flow cytometry analysis detected similar leukocyte levels in the cornea following infection as well. By comparison, there was a significant increase in P. aeruginosa recovered from CXCL1(-/-) corneas as compared with WT mice. Imaging analysis and histochemical staining revealed impaired leukocyte recruitment to the central cornea and earlier corneal thickening in CXCL1(-/-) mice. IFN-γ, CCL2, and CCL5 protein levels were similar between WT and CXCL1(-/-) corneas following HSV-1 or P. aeruginosa infection. However, CXCL2 levels were significantly reduced in the CXCL1(-/-) corneas following either infection.
conclusionsThe absence of CXCL1 and CXCL2 expression significantly impairs the ability of the host to control P. aeruginosa replication through the recruitment of leukocytes to the central cornea. In contrast, CXCL1, CXCL2, and the cells they recruit, are not required for HSV-1 clearance during acute infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.