ArticleJournal of virology2012
Chromatin assembly on herpes simplex virus 1 DNA early during a lytic infection is Asf1a dependent.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Chromatin remodeling by HIRA and FAS2 restricts geminivirus pathogenesis in plants.Virology journal · 2026Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Daxx and HIRA go viral - How chromatin remodeling complexes affect DNA virus infection.Tumour virus research · 2025Review
- The opportunities and challenges of epigenetic approaches to manage herpes simplex infections.Expert review of anti-infective therapy · 2024Review
- Review
- Human cytomegalovirus lytic infection inhibits replication-dependent histone synthesis and requires stem loop binding protein function.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- ATRX limits the accessibility of histone H3-occupied HSV genomes during lytic infection.PLoS pathogens · 2021Article
- Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes.Virology journal · 2021Article
- Herpes simplex virus replication compartments: From naked release to recombining together.PLoS pathogens · 2019Review
- BZLF1 interacts with chromatin remodelers promoting escape from latent infections with EBV.Life science alliance · 2019Article
- ATRX promotes maintenance of herpes simplex virus heterochromatin during chromatin stress.eLife · 2018Article
- Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis.PLoS pathogens · 2018Article
- CTCF interacts with the lytic HSV-1 genome to promote viral transcription.Scientific reports · 2017Article
- Visualizing the replicating HSV-1 virus using STED super-resolution microscopy.Virology journal · 2016Article
- Article
- Identification of small molecules that inhibit the histone chaperone Asf1 and its chromatin function.BMB reports · 2015Article
- Early nucleosome deposition on, and replication of, HSV DNA requires cell factor PCNA.Journal of neurovirology · 2015Article
- Dynamic modulation of HSV chromatin drives initiation of infection and provides targets for epigenetic therapies.Virology · 2015Review
- Genome wide nucleosome mapping for HSV-1 shows nucleosomes are deposited at preferred positions during lytic infection.PloS one · 2015Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Herpes simplex virus (HSV) is a large DNA virus which is characterized by its ability to form latent infections in neurons of the peripheral nervous system. Although histones are found in the capsids of small DNA viruses (papovaviruses), none are found in the capsids of large HSV. However, after entry into the infected cell nucleus, the HSV genome begins to associate with nucleosomes during the earliest stages of infection. In contrast, late during infection, newly replicated viral DNA does not appear to associate with nucleosomes, suggesting that histones are deposited specifically on input viral DNA. The mechanisms of deposition and removing histones from the viral genome are unclear. Recently, histone chaperones, involved in the assembly and disassembly of nucleosomes, have been identified. Human antisilencing factor 1 (Asf1) is one such factor which is involved in both the assembly and disassembly of nucleosomes in cellular systems. In this study, we have examined the effect of small interfering RNA (siRNA) knockdown of Asf1a on HSV infections in HeLa cells. Both viral replication and growth were found to be decreased. Also, viral DNA was significantly less protected from micrococcal nuclease (MNase) digestion up to 6 h postinfection (hpi). However, transcription of the immediate early (IE) genes ICP0 and ICP4 was significantly upregulated at 3 h postinfection. Also, these genes were found to be less protected from MNase digestion and, therefore, less associated with nucleosomes. These results suggest that Asf1a plays a role in regulating IE genes by assembling chromatin onto histone-free viral DNA by 3 h postinfection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.